EXTRACTION AND CHARACTERIZATION OF VERY HIGHLY METHYLATED PECTINS FROM LEMON CELL-WALLS

被引：43

作者：

RALET, MC ^{[1
]}

THIBAULT, JF ^{[1
]}

机构：

[1] INRA,BIOCHIM & TECHNOL GLUCIDES LAB,F-44026 NANTES,FRANCE

来源：

CARBOHYDRATE RESEARCH | 1994年 / 260卷 / 02期

关键词：

D O I：

10.1016/0008-6215(94)84046-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pectins were extracted either by water from extruded lemon fibre or by hot acid from the raw lemon fibre. The amount of water-soluble polysaccharides from lemon plant cell walls was greatly increased after extrusion-cooking. The pectins obtained by extraction with water from the extruded fibre and the pectins extracted from the raw material by hot acid were studied. The water-soluble pectins obtained after extrusion-cooking had the distinctive feature of being very highly (92%) methylated; they were also particularly rich in arabinose side-chains. High molecular weight material coming from the ''hairy'' regions was isolated after digestion by an endo-polygalacturonase. Methylation analysis revealed the presence in both pectins of fairly branched (1 -> 5)-linked arabinans and arabinogalactans of type I and II side-chains.

引用

页码：283 / 296

页数：14

共 53 条

[1]

Ahmed A., 1977, J FOOD BIOCHEM, V1, P361, DOI [DOI 10.1111/J.1745-4514.1978.TB00193.X, 10.1111/j.1745-4514.1978.tb00193.x]

[2]

Asp N. G., 1986, NAHRUNGSFASERN DIETA, P177

[3] LEMON-PEEL PECTIN .2. ISOLATION OF HOMOGENEOUS PECTINS AND EXAMINATION OF SOME ASSOCIATED POLYSACCHARIDES [J].

ASPINALL, GO ;

COTTRELL, IW .

CANADIAN JOURNAL OF CHEMISTRY, 1970, 48 (08) :1283-&

[4] LEMON-PEEL PECTIN .I. FRACTIONATION AND PARTIAL HYDROLYSIS OF WATER-SOLUBLE PECTIN [J].

ASPINALL, GO ;

CRAIG, JWT ;

WHYTE, JL .

CARBOHYDRATE RESEARCH, 1968, 7 (04) :442-&

[5] STRUCTURE OF CITRUS PECTINS AND VISCOMETRIC STUDY OF THEIR SOLUTION PROPERTIES [J].

AXELOS, MAV ;

THIBAULT, JF ;

LEFEBVRE, J .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1989, 11 (03) :186-191

[6] INFLUENCE OF THE SUBSTITUENTS OF THE CARBOXYL GROUPS AND OF THE RHAMNOSE CONTENT ON THE SOLUTION PROPERTIES AND FLEXIBILITY OF PECTINS [J].

AXELOS, MAV ;

THIBAULT, JF .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1991, 13 (02) :77-82

[7] ARABINOGALACTAN PROTEINS FROM STIGMAS OF NICOTIANA-ALATA [J].

BACIC, A ;

GELL, AC ;

CLARKE, AE .

PHYTOCHEMISTRY, 1988, 27 (03) :679-684

[8]

BJORCK I, 1984, CEREAL CHEM, V61, P174

[9] A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS [J].

BLAKENEY, AB ;

HARRIS, PJ ;

HENRY, RJ ;

STONE, BA .

CARBOHYDRATE RESEARCH, 1983, 113 (02) :291-299

[10] A NEW METHOD FOR DETERMINATION OF INSOLUBLE CELL-WALLS AND SOLUBLE NONSTARCHY POLYSACCHARIDES FROM PLANT MATERIALS [J].

BRILLOUET, JM ;

ROUAU, X ;

HOEBLER, C ;

BARRY, JL ;

CARRE, B ;

LORTA, E .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1988, 36 (05) :969-979

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