COMPARISON BETWEEN 2 PROCEDURES FOR STEREOSPECIFIC ANALYSIS OF TRIACYLGLYCEROLS FROM VEGETABLE-OILS .1. OLIVE OIL

Two methods for stereospecific analysis of triacylglycerols are compared. Procedure A, based on stereospecific phosphorylation of sn-1,2 diacylglycerols to phosphatidic acids, and procedure B, based on separation of the diastereomeric 1,2(2,3)-diacylglycerol methane derivatives by high performance liquid chromatography on silica, were applied to olive oil triacyl-sn-glycerols. Statistical evaluation of the results showed good reproducibility, and Student's t-test indicates no statistical differences between the two considered procedures, although some small differences were observed and discussed. Fifteen samples of extra-virgin olive oil, produced in the same region (Umbria, Italy), were analyzed with the two considered procedures.