PURIFICATION AND MODE OF ACTION OF A LOW-MOLECULAR-MASS ENDO-1,4-BETA-D-GLUCANASE FROM FUSARIUM-OXYSPORUM

被引：35

作者：

CHRISTAKOPOULOS, P

KEKOS, D

MACRIS, BJ

CLAEYSSENS, M

BHAT, MK

机构：

[1] INST FOOD RES, READING LAB, DEPT PROT ENGN, READING RG6 2EF, BERKS, ENGLAND

[2] NATL TECH UNIV ATHENS, DEPT CHEM ENGN, ATHENS, GREECE

[3] STATE UNIV GHENT, BIOCHEM LAB, B-9000 GHENT, BELGIUM

来源：

JOURNAL OF BIOTECHNOLOGY | 1995年 / 39卷 / 01期

关键词：

FUSARIUM OXYSPORUM; ENDO-1,4-BETA-D-GLUCANASE; PURIFICATION; MODE OF ACTION; TRANSGLYCOSYLATION;

D O I：

10.1016/0168-1656(94)00147-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A low molecular mass (23.2 kDa) endo-1,4,beta-D-glucanase from Fusarum oxysporum was purified to homogeneity by gel-filtration and ion-exchange chromatographies. The enzyme was optimally active at pH 6.0 and at 50 degrees C. It had a pI value of 8.6 and was stable at 55 degrees C for 1 h. It hydrolyzed carboxymethylcellulose, cello-oligosaccharides (Glc(n)) and 4-methylumbelliferylcello-oligosaccharides but did not hydrolyze cellobiose, p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-xyloside, Avicel, filter paper and xylan. Analysis of reaction mixtures by high pressure liquid chromatography revealed that this enzyme cleaved preferentially the internal glycoside bonds of higher cello-oligosaccharides. The enzyme also catalyzed the formation of transfer products in the presence of cellotriose, cellotetraose and 4-methylumbelliferylglucoside (MeUmbGlc).

引用

页码：85 / 93

页数：9

共 28 条

[1]

[Anonymous], METHOD ENZYMOL

[2] THE CELLULASE OF TRICHODERMA-VIRIDE - PURIFICATION, CHARACTERIZATION AND COMPARISON OF ALL DETECTABLE ENDOGLUCANASES, EXOGLUCANASES AND BETA-GLUCOSIDASES [J].