Swelling-induced catecholamine secretion recorded from single chromaffin cells

被引：26

作者：

Moser, T

Chow, RH

Neher, E

机构：

[1] MAX PLANCK INST BIOPHYS CHEM,ABT MEMBRANBIOPHYS,D-37077 GOTTINGEN,GERMANY

[2] MAX PLANCK INST EXPTL MED,ABT MOLEK BIOL NEURONALER SIGNALE,D-37075 GOTTINGEN,GERMANY

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1995年 / 431卷 / 02期

关键词：

cell swelling; exocytosis; chromaffin cells;

D O I：

10.1007/BF00410191

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

We have studied osmotically induced catecholamine secretion from bovine adrenal chromaffin cells by combining patch-damp measurements, electrochemical detection of secretion, and Fura-2. measurements of intracellular free calcium concentration ([Ca2+](i)). We find that osmotically induced catecholamine release is exocytotic and calcium dependent. Furthermore, we demonstrate that cell swelling is coupled to such secretion via a volume-activated current, carrying predominantly chloride, which causes a plateau depolarization of the cell membrane potential and thus promotes voltage-activated calcium influx. Therefore, cell volume changes may modulate the secretory activity.

引用

页码：196 / 203

页数：8

共 37 条

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