AGE-DEPENDENT CHANGES IN MONKEY LENTICULAR GANGLIOSIDES

被引：9

作者：

OGISO, M ^{[1
]}

SAITO, N ^{[1
]}

SUDO, K ^{[1
]}

HIRANO, S ^{[1
]}

KOMOTO, M ^{[1
]}

机构：

[1] TOHO UNIV,SCH MED,DEPT OPHTHALMOL,TOKYO 143,JAPAN

来源：

EXPERIMENTAL EYE RESEARCH | 1990年 / 50卷 / 01期

关键词：

anti-GM1; antiserum; gangliosides; monkey lens; TLC-immunostaining;

D O I：

10.1016/0014-4835(90)90010-R

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

The content, composition, and distribution of gangliosides were examined in the lenses of normal rhesus monkeys aged 6-16 years. Gangliosides were isolated by organic solvent extraction, DEAE-Sephadex ion-exchange column chromatography, and thin-layer chromatography (TLC). Ganglioside contents determined by the thiobarbituric acid method increased in the lens with aging. TLC analysis of gangliosides showed a much more complex pattern with aging, and the predominant gangliosides were tentatively identified as GM3, GM1, and GD1a. Individual lenticular gangliosides were identified by TLC-immunostaining procedures using anti-GM1 and anti-asialoGM1 antisera. © 1990.

引用

页码：51 / 57

页数：7

共 35 条

[1] HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY AND DENSITOMETRIC DETERMINATION OF BRAIN GANGLIOSIDE COMPOSITIONS OF SEVERAL SPECIES [J].