APPLICATION OF THE IMAGE-ANALYSIS SYSTEM AND IMAGO PROGRAM TO BIOLOGICAL SAMPLES

被引：3

作者：

ARTACHOPERULA, E

ROLDANVILLALOBOS, R

机构：

[1] Department of Morphological Sciences, University of Córdoba, Córdoba, E-14071, Avda. Menendez Pidal s/n

来源：

RESEARCH IN EXPERIMENTAL MEDICINE | 1994年 / 194卷 / 01期

关键词：

IMAGE ANALYSIS SYSTEM; ENDOMETRIUM; ATYPICAL HYPERPLASIA; WELL-DIFFERENTIATED ADENOCARCINOMA; VOLUME-WEIGHTED MEAN NUCLEAR VOLUME;

D O I：

10.1007/BF02576364

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Recent developments in computer systems and new stereological tools have made estimation of quantitative data in biological material simple, easy and fast. The purpose of the present study is to show the advantages and disadvantages of a new program developed by the University of Cordoba for the determination of stereological measurements. As an example, endometrial material was used for demonstrating the technical characteristics. Thus, the nuclear area and volume-weighted mean nuclear volume-(V) over bar(V(nucl))-of 30 routinely processed, paraffin-embedded endometrial specimens from 12 atypical hyperplasias (AH) and 18 well-differentiated adenocarcinomas (WDA) were investigated. We show the excellent visualization of the material and rapid measurement of these quantitative parameters using the Image program. In addition, the mean values of (V) over bar(V(nucl)) are more efficient and useful in separating AH and WDA than the mean nuclear area. It is concluded that (V) over bar(V(nucl)) is a superior and sensitive estimator for discrimination.

引用

页码：35 / 44

页数：10

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