IS THE HUMAN OOCYTE PLASMA-MEMBRANE POLARIZED

被引：52

作者：

SANTELLA, L ^{[1
]}

ALIKANI, M ^{[1
]}

TALANSKY, BE ^{[1
]}

COHEN, J ^{[1
]}

DALE, B ^{[1
]}

机构：

[1] CORNELL UNIV,MED CTR,NEW YORK HOSP,COLL MED,DEPT OBSTET & GYNECOL,CTR REPROD MED & INFERTIL,NEW YORK,NY 10021

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 07期

关键词：

1ST POLAR BODY; REGIONAL MICROMANIPULATION; MICROVILLUS-FREE AREA; ACTIVATION; PARTIAL CORTICAL EXOCYTOSIS;

D O I：

10.1093/oxfordjournals.humrep.a137788

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Using scanning electron microscopy, we have shown that the plasma membrane of the human metaphase II oocyte is organized in evenly spaced, short microvilli of 1-3-mu-m in length. In contrast to other mammals studied to date, there is no microvillus-free area overlying the metaphase spindle and there were no other indications of polarization at-this level of organization. Functional polarity of the plasma membrane, studied using localized microsurgery of the zona pellucida followed by insemination, suggests that sperm fusion and entry in the human may occur anywhere over the oocyte surface. Aged oocytes and those exposed to acidic Tyrode's solution had surfaces which were not homogeneously covered by microvilli. Oocytes exposed to a sucrose solution and subzonally injected with spermatozoa showed evidence of partial cortical granule exocytosis.

引用

页码：999 / 1003

页数：5

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