RECIPROCAL SUBSTITUTIONS ANALYSIS OF EMBRYO INDUCTION AND PLANT-REGENERATION FROM ANTHER CULTURE IN WHEAT (TRITICUM-AESTIVUM L)

被引:13
作者
GHAEMI, M
SARRAFI, A
MORRIS, R
机构
[1] ECOLE NATL SUPER AGRON,INST NATL POLYTECH TOULOUSE,BIOTECHNOL & AMELIORAT PLANTES LAB,F-31076 TOULOUSE,FRANCE
[2] UNIV NEBRASKA,DEPT AGRON,LINCOLN,NE 68583
关键词
ANTHER CULTURE; RECIPROCAL EFFECT; SUBSTITUTION LINES; TRITICUM AESTIVUM; EMBRYOGENESIS;
D O I
10.1139/g95-020
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Reciprocal substitutions for all chromosomes between the hard red winter wheat cultivars Wichita and Cheyenne were used to investigate the effects of individual chromosomes, as well as their interactions with the genetic background, on androgenesis, Duplicate lines for each chromosome were included to check background homogeneity. Six experiments, two for each genome, were performed. In each experiment, 14 substitution lines, their 14 duplicate lines, and the two parental genotypes ('Cheyenne' and 'Wichita') were studied. The experimental design was a randomized block with three replications. 'Wichita' and 'Cheyenne' differed significantly in embryo yield and green plant regeneration (except green plant regeneration for the B-genome tests) and were equal for albino and total plant regeneration. Embryogenesis was influenced by some chromosomes of the A, B, and D genomes; green plant production was influenced by all chromosomes of the A and D genomes except 5D; albino and total plant regeneration were affected by some chromosomes of the B and D genomes. Reciprocal effects were obtained with chromosomes 1A, 7A, 1B, 5B, 1D, and 2D for embryogenesis, chromosomes 2D and 7D for green plant regeneration, and chromosome 2D for total plant regeneration. Reciprocal substitution lines revealed reciprocal effects of homologous chromosomes, as well as interactions between substituted chromosomes and their specific genetic background.
引用
收藏
页码:158 / 165
页数:8
相关论文
共 22 条
[1]   GENETIC-ANALYSIS OF ANTHER CULTURE RESPONSE IN WHEAT USING ANEUPLOID, CHROMOSOME SUBSTITUTION AND TRANSLOCATION LINES [J].
AGACHE, S ;
BACHELIER, B ;
DEBUYSER, J ;
HENRY, Y ;
SNAPE, J .
THEORETICAL AND APPLIED GENETICS, 1989, 77 (01) :7-11
[2]  
ARABI MI, 1991, CEREAL RES COMMUN, V19, P405
[3]   AGRONOMIC PERFORMANCE OF WHEAT DOUBLED-HAPLOID LINES DERIVED FROM CULTIVARS BY ANTHER CULTURE [J].
BAENZIGER, PS ;
WESENBERG, DM ;
SMAIL, VM ;
ALEXANDER, WL ;
SCHAEFFER, GW .
PLANT BREEDING, 1989, 103 (02) :101-109
[4]   ANTHER CULTURE OF WHEAT (TRITICUM-AESTIVUM L) F1S AND THEIR RECIPROCAL CROSSES [J].
BULLOCK, WP ;
BAENZIGER, PS ;
SCHAEFFER, GW ;
BOTTINO, PJ .
THEORETICAL AND APPLIED GENETICS, 1982, 62 (02) :155-159
[5]  
Chuang C. C., 1978, P S PLANT TISSUE CUL, P51
[6]  
DEBUYSER J, 1985, Z PFLANZENZUCHT, V95, P23
[7]  
DEBUYSER J, 1992, PLANT BREEDING, V109, P339, DOI 10.1111/j.1439-0523.1992.tb00194.x
[8]   MEDIUM-GENOTYPE-INTERACTION ON ANDROGENETIC HAPLOID PRODUCTION IN WHEAT [J].
FADEL, F ;
WENZEL, G .
PLANT BREEDING, 1990, 105 (04) :278-282
[9]   THE EFFECT OF THE D GENOME FROM SYNTHETIC WHEAT LINES IN ANTHER CULTURE RESPONSES [J].
GHAEMI, M ;
SARRAFI, A .
PLANT BREEDING, 1994, 112 (01) :76-79
[10]   INFLUENCE OF GENOTYPE AND CULTURE CONDITIONS ON THE PRODUCTION OF EMBRYOS FROM ANTHERS OF TETRAPLOID WHEAT (TRITICUM-TURGIDUM) [J].
GHAEMI, M ;
SARRAFI, A ;
ALIBERT, G .
EUPHYTICA, 1993, 65 (02) :81-85