STRUCTURE OF TYROSINE PHENOL-LYASE GENES FROM CITROBACTER-FREUNDII AND STRUCTURAL COMPARISON WITH TRYPTOPHANASE FROM ESCHERICHIA-COLI

被引：17

作者：

IWAMORI, S

YOSHINO, S

ISHIWATA, K

MAKIGUCHI, N

机构：

[1] Life Science Laboratory, Mitsui Toatsu Chemicals Inc., Mobara-shi, Chiba, 297

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1991年 / 72卷 / 03期

关键词：

D O I：

10.1016/0922-338X(91)90207-W

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The tyrosine phenol-lyase (TPL) gene of Citrobacter freundii was cloned and the complete nucleotide sequence of the TPL gene was determined. The TPL gene consists of 1368bp (456 amino acids). The deduced amino acid sequence of TPL was compared with that of tryptophanase from Escherichia coli. A lot of regions are highly conserved, and total homology was 44%. From the observation of the homology data, the PLP binding site of TPL was predicted and confirmed by site-directed mutagenesis.

引用

页码：147 / 151

页数：5

共 26 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] SEQUENCE SIMILARITIES BETWEEN TRYPTOPHAN SYNTHASE-BETA SUBUNIT AND OTHER PYRIDOXAL-PHOSPHATE-DEPENDENT ENZYMES [J].