HYDRALAZINE STIMULATES PRODUCTION OF OXYGEN FREE-RADICALS IN EAGLES MEDIUM AND CULTURED FIBROBLASTS

被引：21

作者：

WEGLARZ, L

BARTOSZ, G

机构：

[1] Department of Biochemistry and Chemistry, Silesian Medical Academy, 40-752 Katowice

[2] ódź, Łódź

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1991年 / 11卷 / 02期

关键词：

HYDRALAZINE; FIBROBLASTS; ESR; SPIN TRAPPING; DMPO; TIRON; HYDROXYL RADICAL; SUPEROXIDE RADICAL ANION; FREE RADICALS;

D O I：

10.1016/0891-5849(91)90165-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of hydralazine on the oxygen free radical production was studied in whole cultured murine liver fibroblasts and mitochondrial and microsomal fractions of the cells by ESR spin trapping with DMPO and measurement of Tiron semiquinone formation. Hydralazine itself was found to generate free radicals in phosphate buffer and especially in Eagle's Minimal Essential Medium. Most of the adduct of the spin trap DMPO was due to its reaction with hydralazine-induced hydroxyl radical. Moreover, this compound stimulated free radical formation in fibroblasts. These data suggest that hydralazine alters the cellular free radical metabolism which may have implications for the biological activity of this drug.

引用

页码：149 / 155

页数：7

共 21 条

[1]

Alarcon-Segovia, Wakim, Worthington, Work, Clinical and experimental studies on the hydralazine syndrome and its relationship to systemic lupus erythematosus, Medicine, 46, pp. 1-33, (1967)

[2]

Jendryczko, Drozdz, The proline level and the collagen metabolism in patients with generalized erythematosus lupus, Przeglad Lek., 41, pp. 563-565, (1984)

[3]

Drozdz, Kucharz, Glowacki, Glycosaminoglycan content in tissues and body fluids of rats with drug-induced collagen-like syndrome, Rev. Roum. Biochim., 20, pp. 241-249, (1983)

[4]

Drozdz, Weglarz, Wardas, Kula, Biochemical changes in cultured murine fibroblasts after treatment with hydrazinophtalazines, Clin. Physiol. Biochem., 7, pp. 97-100, (1989)

[5]

Misra, Fridovich, The oxidation of phenylhydrazine: superoxide and mechanism, Biochemistry, 15, pp. 681-687, (1976)

[6]

Hill, Thornalley, Phenyl radical production during the oxidation of phenylhydrazine and in phenylhydrazine-induced haemolysis, FEBS Lett., 125, pp. 235-238, (1981)

[7]

Augusto, Ortiz de Montellano, Quintanilha, Spin-trapping of free radicals formed during microsomal metabolism of ethyl hydrazine and acetylhydrazine, Biochem. Biophys. Res. Commun., 10, pp. 1324-1330, (1981)

[8]

Sinha, Motten, Oxidative metabolism of hydralazine. Evidence for nitrogen centered radicals formation, Biochem. Biophys. Res. Commun., 105, pp. 1044-1053, (1982)

[9]

Sinha, Enzymatic activation of hydralazine derivatives, J. Biol. Chem., 258, pp. 796-801, (1983)

[10]

Weglarz, Drozdz, Wardas, Goss, Activities of antioxidant enzymes in fibroblasts cultured in vitro in the presence o hydralazine, Biomed. Biochim. Acta, 478, pp. 489-493, (1989)

← 1 2 3 →