TRANSIENT-RESPONSE METHOD FOR EVALUATING THE RATE CONSTANTS OF REACTIONS OVER IMMOBILIZED ENZYMES

被引：6

作者：

WU, JY ^{[1
]}

WENG, HS ^{[1
]}

机构：

[1] NATL CHENG KUNG UNIV,DEPT CHEM ENGN,TAINAN 70101,TAIWAN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1991年 / 37卷 / 10期

关键词：

TRANSIENT RESPONSE METHOD; RATE CONSTANTS; IMMOBILIZED ENZYME; GLUCOSE OXIDATION;

D O I：

10.1002/bit.260371005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The transient response method was utilized to evaluate the rate constants of reaction over immobilized enzyme. Glucose oxidation catalyzed by the immobilized glucose oxidase in a fixed-bed reactor was selected as an example. A theoretical model including the effects of axial dispersion, film diffusion, and intraparticle diffusion was established for the reactor. The individual rate constant of each elementary step of this enzymatic reaction was determined through direct fitting of the experimental response data to the model.

引用

页码：922 / 926

页数：5

共 10 条

[1]

Beveridge G., 1970, OPTIMIZATION THEORY

[2] PREDICTION OF BREAKTHROUGH CURVES BY THE APPLICATION OF FAST FOURIER-TRANSFORM [J].