DISSOCIATION BY NH4CL TREATMENT OF THE ENZYMATIC-ACTIVITIES OF GLUTAMINE SYNTHETASE-II FROM RHIZOBIUM-LEGUMINOSARUM BIOVAR VICEAE

被引:16
作者
MANCO, G
ROSSI, M
DEFEZ, R
LAMBERTI, A
PERCUOCO, G
IACCARINO, M
机构
[1] CNR, INT INST GENET & BIOPHYS, VIA MARCONI 12, I-80125 NAPLES, ITALY
[2] CNR, INST BIOCHEM PROT & ENZYMOL, I-80125 NAPLES, ITALY
来源
JOURNAL OF GENERAL MICROBIOLOGY | 1992年 / 138卷
关键词
D O I
10.1099/00221287-138-7-1453
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Glutamine synthetase II (GSII) was purified to homogeneity from Rhizobium leguminosarum biovar viceae and characterized. The sequence of 26 amino acid residues from the amino-terminal end of the protein showed high similarity with the sequence of GSII from Bradyrhizobium japonicum or from Rhizobium meliloti. Non-denaturing PAGE showed that GSII, either in crude extracts or in the pure state, was a mixture of an octamer and a tetramer and that under specific conditions the octamer/tetramer ratio could be modified in either direction. The pure enzyme was used to raise an antiserum which was highly specific. Addition of NH4Cl to a bacterial culture derepressed for GSII caused a specific decrease in transferase activity, faster than the one observed when the amount of immunoreactive material was measured by different methods. On the other hand, biosynthetic activity, measured as the rate of ADP or glutamine formation, paralleled the rate of decrease in immunoreactive material. A partially purified enzyme preparation retained this dissociation of kinetic parameters, strongly suggesting a post-translational modification. These findings are discussed with respect to the possible role of GSII in the Rhizobium-legume symbiosis.
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页码:1453 / 1460
页数:8
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