PHOSPHORYLATION OF SER-42 AND SER-59 IN THE N-TERMINAL REGION OF THE TYROSINE KINASE P56(LCK)

被引：90

作者：

WINKLER, DG ^{[1
]}

PARK, I ^{[1
]}

KIM, T ^{[1
]}

PAYNE, NS ^{[1
]}

WALSH, CT ^{[1
]}

STROMINGER, JL ^{[1
]}

SHIN, J ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL,BOSTON,MA 02115

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 11期

关键词：

SERINE PHOSPHORYLATION; PHORBOL ESTER; MITOGEN-ACTIVATED PROTEIN KINASE; PROTEIN KINASE-C;

D O I：

10.1073/pnas.90.11.5176

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ser-42 and Ser-59 in the N-terminal region have been identified as the major phorbol ester-induced phosphorylation sites of p56lck. Phosphorylation of Ser-59 results in a gel shift from 56 kDa to 61 kDa. Simultaneous phosphorylation of Ser-42 and Ser-59 results in a further gel shift to 63 kDa. In vitro kinase assays show that Ser-59 can be uniquely phosphorylated by mitogen-activated protein kinase and that Ser-42 can be phosphorylated by either protein kinase A or protein kinase C.

引用

页码：5176 / 5180

页数：5

共 28 条

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