BASOLATERAL PROTEIN-TRANSPORT IN STREPTOLYSIN O-PERMEABILIZED MDCK CELLS

被引:72
作者
PIMPLIKAR, SW [1 ]
IKONEN, E [1 ]
SIMONS, K [1 ]
机构
[1] EUROPEAN MOLEC BIOL LAB,CELL BIOL PROGRAMME,D-69012 HEIDELBERG,GERMANY
关键词
D O I
10.1083/jcb.125.5.1025
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have reconstituted polarized protein transport in streptolysin O-permeabilized MDCK cells from the TGN to the basolateral surface and to the apical surface. These transport steps are dependent on temperature, energy and exogenously supplied cytosol. Using this in vitro system we show that a whole tail peptide (WT peptide) corresponding to the cytoplasmic tail of a basolaterally sorted protein, the vesicular stomatitis virus glycoprotein (VSV G) inhibits the TGN to basolateral transport but does not affect any other transport step. Inhibition of VSV G transport to basolateral surface by WT peptide did not result in missorting of the protein to the apical surface. Mutation of the single tyrosine residue in the WT peptide reduced its inhibitory potency four- to fivefold. These results suggest that the VSV G tail physically interacts with a component of the sorting machinery. Using a cross-linking approach, we have identified proteins that associate with the cytoplasmic tail domain of VSV G. One of these polypeptides, Tin-2 (Tail interacting protein-2), associates with VSV G in the TGN, the site of protein sorting, but not in the ER nor at the cell surface. Tin-2 does not associate with apically targeted hemagglutinin. WT peptide that inhibited the basolateral transport of VSV G also inhibited the association of Tin-2 with VSV G. Together, these properties make Tin-2 a candidate basolateral sorter. The results demonstrate the usefulness of the SLO-permeabilized cell system in dissecting the sorting machinery.
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页码:1025 / 1035
页数:11
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