FORMATION OF THE EXTRACELLULAR PROTEASES FROM TRICHODERMA-REESEI QM-9414 INVOLVED IN CELLULASE DEGRADATION

被引：48

作者：

HAAB, D ^{[1
]}

HAGSPIEL, K ^{[1
]}

SZAKMARY, K ^{[1
]}

KUBICEK, CP ^{[1
]}

机构：

[1] VIENNA TECH UNIV,INST BIOCHEM TECHNOL & MIKROBIOL,MIKROBIELLE BIOCHEM ABT,A-1060 VIENNA,AUSTRIA

来源：

JOURNAL OF BIOTECHNOLOGY | 1990年 / 16卷 / 3-4期

关键词：

Acid protease; Cellulase degradation; Trichoderma reesei;

D O I：

10.1016/0168-1656(90)90035-A

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The production and properties of a protease of Trichoderma reesei QM 9414, which is secreted during growth on cellulose, was investigated: formation of protease activity was mainly induced by the presence of organic nitrogen in the medium, and only little affected by changes in the pH between 3 and 5. Variations in the carbon source were without effect. High levels of protease in the extracellular culture fluid correlated with the appearance of proteolytic cellulase degradation products. The major protease was partially purified and shown to resemble a pepstatin-insensitive, N-chlorosuccinimide sensitive aspartate protease. © 1990.

引用

页码：187 / 198

页数：12

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