IDENTIFICATION AND PARTIAL CHARACTERIZATION OF PHOSPHOLIPASE-D IN THE HUMAN AMNIOTIC MEMBRANE

被引：14

作者：

INAMORI, K ^{[1
]}

SAGAWA, N ^{[1
]}

HASEGAWA, M ^{[1
]}

ITOH, H ^{[1
]}

UEDA, H ^{[1
]}

KOBAYASHI, F ^{[1
]}

IHARA, Y ^{[1
]}

MORI, T ^{[1
]}

机构：

[1] KYOTO UNIV,FAC MED,DEPT GYNECOL & OBSTET,54 KAWAHARA CHO,SHOGOIN,SAKYO KU,KYOTO 606,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 191卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1354

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzymatic activity of phospholipase D and its characteristics have been examined in human amnion tissue. The phospholipase D activity was not Ca2+- or Mg2+-dependent and was activated by unsaturated fatty acids. The optimal pH of phospholipase D was 5.5. The phospholipase D activity in amnion tissue was highest in the microsomal fraction, and preferentially utilized phosphatidylcholine as a substrate. The phospholipase D activity of the microsomal fraction of amnion tissue obtained at term before labor onset (34.0 ± 16.3 nmol/hour/mg protein, mean ± SD, n=11) was significantly (p<0.05) higher than the activity in this tissue obtained from women in the mid-trimester (15.0 ± 7.5 nmol/hour/mg protein, n=9). © 1993 Academic Press.

引用

页码：1270 / 1277

页数：8

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