REACTION-RATES IN ORGANIC MEDIA SHOW SIMILAR DEPENDENCE ON WATER ACTIVITY WITH LIPASE CATALYST IMMOBILIZED ON DIFFERENT SUPPORTS

被引：16

作者：

OLADEPO, DK ^{[1
]}

HALLING, PJ ^{[1
]}

LARSEN, VF ^{[1
]}

机构：

[1] UNIV STRATHCLYDE,DEPT CHEM & PROC ENGN,GLASGOW G1 1XW,SCOTLAND

来源：

BIOCATALYSIS | 1994年 / 8卷 / 04期

关键词：

SUPPORT MATERIAL; IMMOBILIZATION; LIPASE; REACTION RATES; WATER CONTENT; WATER ACTIVITY;

D O I：

10.3109/10242429408998212

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipase (E.C. 3.1.1.3) from Rhizomucor miehei was adsorbed on silica, zirconia and five alumina support materials. The immobilised preparations were used to catalyse esterification reactions of decanoic acid and dodecanol in hexane. The immobilised lipase and the organic phase were separately preequilibrated to the desired water activities. The various support materials adsorbed widely different amounts of water at a given water activity. The reaction rates with all the support materials show similar dependence on water activity when the rates were normalised with the optimal rate for that support material. Hence water activity predicts the optimal conditions much better than water content.

引用

页码：283 / 287

页数：5

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