A NEW TYPE POROUS CARRIER AND ITS APPLICATION TO CULTURE OF SUSPENSION CELLS

被引：11

作者：

GOTOH, T ^{[1
]}

HONDA, H ^{[1
]}

SHIRAGAMI, N ^{[1
]}

UNNO, H ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,DEPT BIOENGN,4259 NAGATSUTA CHO,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

CYTOTECHNOLOGY | 1993年 / 11卷 / 01期

关键词：

POROUS CARRIER; SUSPENSION-CELL CULTURE;

D O I：

10.1007/BF00749055

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new type porous carrier was fabricated from a mixture of sodium alginate, bovine serum albumin and sodium bicarbonate. The porous space of the carrier is an assembly of void spaces. The carrier was successfully applied to the cultivation of suspension animal cells. In the culture, while both cells and carriers were held in suspension, the cells were entrapped hydrodynamically into the void spaces in the carriers. A culture of hybridoma cells using this carrier resulted in a cell density up to 5.7 x 10(7) cells per ml-carrier.

引用

页码：35 / 40

页数：6

共 10 条

[1] ENCAPSULATION OF VIABLE CELLS WITHIN POLYACRYLATE MEMBRANES [J].