RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF ETHYNYLOESTRADIOL IN RABBIT PLASMA

被引:15
作者
FERNANDEZ, N
GARCIA, JJ
DIEZ, MJ
TERAN, MT
SIERRA, M
机构
[1] Department of Physiology, Pharmacology and Toxicology, Veterinary Faculty, University of León
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 619卷 / 01期
关键词
D O I
10.1016/0378-4347(93)80458-G
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A method for the determination of ethynyloestradiol in samples of rabbit plasma containing pentobarbital and heparin, the former used as an anaesthetic and the latter as an anticoagulant, has been developed. Quantification was carried out using a reversed-phase high-performance liquid chromatographic (HPLC) method in isocratic mode at room temperature, with electrochemical detection at an applied potential of +1 V vs. Ag/AgCl. Under these conditions, the retention time for ethynyloestradiol was ca. 2.9 min, the average recovery from plasma was 74.5%, and the limit of detection was 10 pg, corresponding to a plasma concentration of 50 pg/ml using 1 ml of plasma. Natural oestrogens, oestriol, oestradiol and oestrone showed peaks that did not interfere with ethynyloestradiol, and retention times of ca. 0.8, 2.4 and 3.4 min, respectively.
引用
收藏
页码:143 / 147
页数:5
相关论文
共 12 条
[1]   ASSAY OF ETHYNYLOESTRADIOL IN HUMAN-SERUM AND ITS BINDING TO PLASMA-PROTEINS [J].
AKPOVIRORO, J ;
FOTHERBY, K .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1980, 13 (07) :773-779
[2]   INVESTIGATION OF THE PHARMACOKINETICS OF ETHYNYLESTRADIOL IN WOMEN USING RADIOIMMUNOASSAY [J].
BACK, DJ ;
BRECKENRIDGE, AM ;
CRAWFORD, FE ;
MACIVER, M ;
ORME, MLE ;
ROWE, PH ;
WATTS, MJ .
CONTRACEPTION, 1979, 20 (03) :263-273
[3]   DETERMINATION OF ETHINYLESTRADIOL IN SINGLE TABLETS AND ITS SEPARATION FROM OTHER STEROIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
BAGON, KR ;
HAMMOND, EW .
ANALYST, 1978, 103 (1223) :156-161
[4]   QUANTITATIVE-ANALYSIS OF ETHYNODIOL DIACETATE AND ETHINYL ESTRADIOL MESTRANOL IN ORAL-CONTRACEPTIVE TABLETS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
CARIGNAN, G ;
LODGE, BA ;
SKAKUM, W .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1982, 71 (02) :264-266
[5]   MEASUREMENT OF ETHYNYLOESTRADIOL BY RADIOIMMUNOASSAY AND BY ISOTOPE DILUTION-MASS SPECTROMETRY [J].
FOTHERBY, K ;
AKPOVIRORO, JO ;
SIEKMANN, L ;
BREUER, H .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1981, 14 (05) :499-500
[6]   INVESTIGATIONS OF PHARMACOKINETICS OF ETHINYLESTRADIOL TO SPECIFIC CONSIDERATION OF A POSSIBLE 1ST-PASS EFFECT IN WOMEN [J].
HUMPEL, M ;
NIEUWEBOER, B ;
WENDT, H ;
SPECK, U .
CONTRACEPTION, 1979, 19 (04) :421-432
[7]   PLASMA-LEVELS OF ETHINYLESTRADIOL (EE) DURING CYCLIC TREATMENT WITH COMBINED ORAL-CONTRACEPTIVES [J].
KAUFMAN, JM ;
THIERY, M ;
VERMEULEN, A .
CONTRACEPTION, 1981, 24 (05) :589-602
[8]   DETERMINATION OF NORGESTIMATE AND ETHINYL ESTRADIOL IN TABLETS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
LANE, PA ;
MAYBERRY, DO ;
YOUNG, RW .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1987, 76 (01) :44-47
[9]   DISSOLUTION TESTING OF NORETHINDRONE-ETHINYL ESTRADIOL, NORETHINDRONE-MESTRANOL, AND NORETHINDRONE ACETATE-ETHINYL ESTRADIOL COMBINATION TABLETS [J].
NGUYEN, HT ;
SHIU, GK ;
WORSLEY, WN ;
SKELLY, JP .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1990, 79 (02) :163-167
[10]   RADIOIMMUNOASSAY OF PLASMA ETHINYLESTRADIOL IN THE PRESENCE OF CIRCULATING NORETHINDRONE [J].
STANCZYK, FZ ;
GALE, JA ;
GOEBELSMANN, U ;
NERENBERG, C ;
MATIN, S .
CONTRACEPTION, 1980, 22 (05) :457-470