HOW 434-REPRESSOR DISCRIMINATES BETWEEN O(R)1 AND O(R)3 - THE INFLUENCE OF CONTACTED AND NONCONTACTED BASE-PAIRS

被引:13
作者
BELL, AC [1 ]
KOUDELKA, GB [1 ]
机构
[1] SUNY BUFFALO,DEPT SCI BIOL,BUFFALO,NY 14260
关键词
D O I
10.1074/jbc.270.3.1205
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The sequence of the bacteriophage 434 O(R)1 (ACAAAACTTTCTTGT) differs from its O(R)3 (ACAGTTTTTCTTGT) at positions 4-6. X-ray analysis shows that the side chain of Gln(33) of the 434 repressor makes van der Waals' and H-bond contacts with the T at position 4' in complex with O(R)1, but no specific contact is observed at this position in 434 repressor-O(R)3 complexes, No contacts are made by repressor to the bases at positions 5 or 6 in either binding site, The significance of the sequence differences between O(R)1 and O(R)3 in determining the operator affinity for repressor were examined by con structing synthetic variants of these operators, Measurements of the affinity of these operators for repressor as a function of ionic strength revealed that although base pairs 5 and 6 are not contacted by 434 repressor, they can nonetheless influence operator affinity for repressor by modulating the degree to which ionic interactions contribute to the overall binding energy. Both the magnitude and direction of their effect depends on the status of repressor's contacts to the bases at position 4. The role of contact made by Gln(33) to position 4 was examined by mutating this amino acid to Ala and by examining the affinity of wild type repressor for an operator bearing a 5-methylcytosine at position 4' in an O(R)1-4G mutant. These experiments showed that repressor's preferences at operator positions 5 and 6 are linked to its position 4 preference via a van der Waals' contact between amino acid 33 and a methyl group on the base at operator position 4'. Together, the results of the experiments shown here reveal that bases that do not contact the protein alter its preferences for bases at the contacted operator position 4.
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页码:1205 / 1212
页数:8
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