INACTIVATION OF HUMAN PLASMA ALPHA-1-PROTEINASE INHIBITOR BY HUMAN-PMN LEUKOCYTE COLLAGENASE

被引：58

作者：

KNAUPER, V ^{[1
]}

REINKE, H ^{[1
]}

TSCHESCHE, H ^{[1
]}

机构：

[1] UNIV BIELEFELD, FAC CHEM, DEPT BIOCHEM, POB 8640, UNIV STR, W-4800 BIELEFELD 1, GERMANY

来源：

FEBS LETTERS | 1990年 / 263卷 / 02期

关键词：

Polymorphonuclear leucocyte collagenase; Proteolytic inactivation; α[!sub]1[!/sub]-Proteinase inhibitor;

D O I：

10.1016/0014-5793(90)81412-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Highly purified human polymorphonuelear leucocyte collagenase cleaved human α-1-proteinase inhibitor (α1-PI) at the carboxyl site of Phe352 (P7). The inhibitor was thereby rapidly inactivated and generated a primary degradation product as shown by reverse-phase HPLC and N-terminal sequencing. Prolonged incubation of the modified inhibitor with polymorphonuclear leucocyte collagenase led to the generation of a secondary degradation product with additional cleavage at the carboxyl site of Pro357 (P2). © 1990.

引用

页码：355 / 357

页数：3

共 25 条

[1] REGULATION OF ALPHA-1 PROTEINASE-INHIBITOR FUNCTION BY RABBIT ALVEOLAR MACROPHAGES - EVIDENCE FOR PROTEOLYTIC RATHER THAN OXIDATIVE INACTIVATION [J].