MULTIPLE PRIMER PAIRS POLYMERASE CHAIN-REACTION FOR THE DETECTION OF HUMAN PAPILLOMAVIRUS TYPES

被引：16

作者：

ANCESCHI, MM

FALCINELLI, C

PIERETTI, M

COSMI, EV

机构：

[1] Istituto di Ginecologia ed Ostetricia, Policlinico Monteluce, Perugia

来源：

JOURNAL OF VIROLOGICAL METHODS | 1990年 / 28卷 / 01期

关键词：

Human papillomavirus; Multiple primer pairs; Polymerase chain reaction;

D O I：

10.1016/0166-0934(90)90087-V

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A polymerase chain reaction (PCR) based on the use of multiple primers enabling the simultaneous detection of HPV-6b, -11, -16 and -18 in a single tube reaction was developed and validated on cervico-vaginal specimens, including tissues embedded in paraffin. This PCR setting proved to be specific and sensitive, allowing the detection of as few as 200 viral particles per specimen. © 1990.

引用

页码：59 / 66

页数：8

共 10 条

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