RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS IN THE RIBOSOMAL GENE SPACERS OF TRYPANOSOMA-CRUZI AND TRYPANOSOMA-CONORHINI

被引：21

作者：

DIETRICH, P ^{[1
]}

DUSSAN, MD ^{[1
]}

FLOETERWINTER, LM ^{[1
]}

AFFONSO, MHT ^{[1
]}

CAMARGO, EP ^{[1
]}

SOARES, MB ^{[1
]}

机构：

[1] UNIV SAO PAULO, DEPT PARASITOL, SAO PAULO, SP, BRAZIL

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1990年 / 42卷 / 01期

关键词：

Restriction fragment length polymorphism; Taxonomy; Trypanosoma conorhini rRNA genes; Trypanosoma cruzi rRNA genes;

D O I：

10.1016/0166-6851(90)90108-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ribosomal RNA genes of two species of Trypanosoma, Trypanosoma cruzi, the etiological agent of Chagas' disease, and Trypanosoma conorhini, a non-pathogenic rodent trypanosome, were cloned and partially characterized. The physical maps derived for their rRNA genes were similar throughout the region that encompasses the SSU- and LSU-rRNA coding sequences. However, the non-transcribed spacer DNA of both T. cruzi and T. conorhini was found to be polymorphic for several restriction enzyme sites. We show that strains of T. cruzi can be typed according to the characteristic restriction fragment length polymorphism of their NTS DNAs. © 1990.

引用

页码：13 / 19

页数：7

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