ACTIONS OF VASOPRESSIN AND ISOPRENALINE ON THE IONIC TRANSPORT ACROSS THE ISOLATED FROG-SKIN IN THE PRESENCE AND THE ABSENCE OF ADENYL-CYCLASE INHIBITOR-MDL12330A AND INHIBITOR-SQ22536

被引：68

作者：

LIPPE, C ^{[1
]}

ARDIZZONE, C ^{[1
]}

机构：

[1] UNIV BARI,DEPT PHARMACOBIOL,I-70124 BARI,ITALY

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY | 1991年 / 99卷 / 1-2期

关键词：

D O I：

10.1016/0742-8413(91)90101-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. The effects of both adenyl cyclase inhibitors (MDL12330A and SQ22536) have been studied on the ionic transport induced by vasopressin and isoprenaline across the frog skin. 2. MDL12330A inhibits the vasopressin action on the short-circuit current (SCC), confirming that this effect is cAMP-mediated. 3. On the other hand, isoprenaline action on the SCC is unaffected by MDL12330A. However, this lack of effect is not a sufficient argument against the role of cAMP in this action; in fact, as MDL12330A is also an inhibitor of cAMP phosphodiesterase, this action could mask the inhibitory effect of the drug on adenyl cyclase. 4. By using the other adenyl cyclase inhibitor (SQ22536), probably deprived of effect on the cAMP phosphodiesterase, we obtained a strong inhibition of isoprenaline action on the SCC. Thus we conclude that the actions of isoprenaline on the ionic transport across the frog skin are also cAMP-mediated.

引用

页码：209 / 211

页数：3

共 7 条

[1]

GUGGINO WB, 1989, SCIENCE, V244, P1351

[2] INHIBITION ADENYLATE-CYCLASE BY ADENOSINE-ANALOGS IN PREPARATIONS OF BROKEN AND INTACT HUMAN PLATELETS - EVIDENCE FOR UNIDIRECTIONAL CONTROL OF PLATELET-FUNCTION BY CYCLIC-AMP [J].