ATOMIC MODEL OF A PYRIMIDINE DIMER EXCISION-REPAIR ENZYME COMPLEXED WITH A DNA SUBSTRATE - STRUCTURAL BASIS FOR DAMAGED DNA RECOGNITION

被引：242

作者：

VASSYLYEV, DG ^{[1
]}

KASHIWAGI, T ^{[1
]}

MIKAMI, Y ^{[1
]}

ARIYOSHI, M ^{[1
]}

IWAI, S ^{[1
]}

OHTSUKA, E ^{[1
]}

MORIKAWA, K ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

CELL | 1995年 / 83卷 / 05期

关键词：

D O I：

10.1016/0092-8674(95)90190-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

T4 endonuclease V is a DNA repair enzyme from bacteriophage T4 that catalyzes the first reaction step of the pyrimidine dimer-specific base excision repair pathway. The crystal structure of this enzyme complexed with a duplex DNA substrate, containing a thymine dimer, has been determined at 2.75 Angstrom resolution, The atomic structure of the complex reveals the unique conformation of the DNA duplex, which exhibits a sharp kink with a 60 degrees inclination at the central thymine dimer, The adenine base complementary to the 5' side of the thymine dimer is completely flipped out of the DNA duplex and trapped in a cavity on the protein surface. These structural features allow an understanding of the catalytic mechanism and implicate a general mechanism of how other repair enzymes recognize damaged DNA duplexes.

引用

页码：773 / 782

页数：10

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