3-DIMENSIONAL MAMMALIAN-CELL GROWTH ON NONWOVEN POLYESTER FABRIC DISKS

被引：13

作者：

PETTI, SA ^{[1
]}

LAGES, AC ^{[1
]}

SUSSMAN, MV ^{[1
]}

机构：

[1] TUFTS UNIV,DEPT CHEM ENGN,MEDFORD,MA 02155

来源：

BIOTECHNOLOGY PROGRESS | 1994年 / 10卷 / 05期

关键词：

D O I：

10.1021/bp00029a015

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Small disks of nonwoven polyester fabric (NWPF), similar to Fibra-Cel carriers, were surface treated with strong acid and used as a high surface area support matrix for in vitro culture of anchorage dependent MRC-5 cells. The disks can be autoclaved, and require inocula concentrations of only 2-5% of the final cell densities, which have reached 10(8) cells/mL of disk bed volume. Scanning electron microscopy photographs shown herein re veal that cells grow in a multilayered fashion between the randomly arrayed fibers of the nonwoven fabric, emulating in vivo growth.

引用

页码：548 / 550

页数：3

共 12 条

[1]

BAKER DJ, 1990, THESIS TUFTS U MEDFO

[2]

BAKER DJ, 1991, INDA J, V3, P44

[3]

BOHAK Z, 1989, Patent No. 2178447

[4]

FELDMAN AF, 1986, THESIS TUFTS U MEDFO

[5]

KADOURI A, 1987, BIOPOLYMERS, V26, pS205

[6]

KADOURI A, 1989, MAY INT NONW FABR C, P477

[7] USE OF A STATIONARY BED REACTOR AND SERUM-FREE MEDIUM FOR THE PRODUCTION OF RECOMBINANT PROTEINS IN INSECT CELLS [J].

KOMPIER, R ;

KISLEV, N ;

SEGAL, I ;

KADOURI, A .

ENZYME AND MICROBIAL TECHNOLOGY, 1991, 13 (10) :822-827

[8]

Mirchamsy H, 1976, Dev Biol Stand, V37, P297

[9]

PETTII S, 1993, THESIS TUFTS U MEDFO

[10]

VERDON SL, 1990, SCANNING MICROSCOPY, V4, P341

← 1 2 →