SENSITIVE PULSED AMPEROMETRIC DETECTION OF FREE AND CONJUGATED BILE-ACIDS IN COMBINATION WITH GRADIENT REVERSED-PHASE HPLC

被引：33

作者：

DEKKER, R ^{[1
]}

VANDERMEER, R ^{[1
]}

OLIEMAN, C ^{[1
]}

机构：

[1] NETHERLANDS INST DAIRY RES, POB 20, 6710 BA EDE, NETHERLANDS

来源：

CHROMATOGRAPHIA | 1991年 / 31卷 / 11-12期

关键词：

LIQUID CHROMATOGRAPHY; BILE ACIDS; GLYCO-CONJUGATES; TAURO-CONJUGATES; ELECTROCHEMICAL DETECTION;

D O I：

10.1007/BF02279474

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and sensitive method has been developed for the simultaneous determination of free bile acids and its glyco- and tauro-conjugates by high-performance liquid chromatography (HPLC). These components were separated on a resin-based reversed-phase column, with a gradient of acetonitrile in dilute sodium hydroxide solution. The detection was performed at a gold electrode with a sequence of three pulses, which enabled the selective detection of hydroxyl-containing compounds, without fouling of the electrode. Detection limits ranged from 30 to 60 pmol on-column. Linear calibration graphs were obtained for bile acid solutions of 0.6 to 14-mu-mol/l. The method was applied to the determination of bile acids in culture media, bile, and ileal fluid.

引用

页码：549 / 553

页数：5

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