FACILITATED NUCLEAR TRANSPORT OF CALMODULIN IN TISSUE-CULTURE CELLS

被引:61
作者
PRUSCHY, M
JU, Y
SPITZ, L
CARAFOLI, E
GOLDFARB, DS
机构
[1] UNIV ROCHESTER,DEPT BIOL,ROCHESTER,NY 14627
[2] ETH ZURICH,INST BIOCHEM 3,CH-8092 ZURICH,SWITZERLAND
关键词
D O I
10.1083/jcb.127.6.1527
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Calmodulin (CaM) potentiates Ca2+-dependent signaling pathways in both the cytoplasm and nucleus. We have investigated the mechanism of CaM nuclear transport using tissue culture cell microinjection and a permeabilized cell import assay. The inhibition of CaM import by the translocation inhibitor wheat germ agglutinin (WGA) and by chilling, indicates that CaM import is facilitated, but because ATP depletion does not affect CaM import, the mechanism does not appear to be active. Chilling and WGA arrest persist in ATP-depleted cells, indicating that CaM is not retained in the cytoplasm by an ATP-dependent mechanism. In permeabilized cells, both Ca2+-CaM and Ca2+-free CaM are sensitive to extract-dependent WGA and chilling import inhibition. Titration experiments in microinjected and permeabilized cells indicate that a saturable cytosolic factor(s) mediates chilling and WGA arrest.
引用
收藏
页码:1527 / 1536
页数:10
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