EXPRESSION OF GROWTH-FACTORS IN EARLY WOUND-HEALING IN RAT SKIN

Growth factors are a group of hormone-like polypeptides that have been shown to play a central role in different phases of wound healing. The expression of these growth factors in early wound healing has not been quantified, and the pattern and distribution of these growth factors in early wound healing has not been described completely. Furthermore the quantity and pattern of distribution of these growth factors have not been investigated in early wounds produced by various methods of surgical incision. Comparison of the rate of healing between the CO2 laser wound and the scalpel wound has produced conflicting results. The present immunohistochemical study uses polyclonal antibodies specific for epidermal growth factor (FGF), platelet-derived growth factor (PDGF), transforming growth factor beta (TGF-beta), and basic fibroblast growth factor (bFGF) to observe the pattern and distribution of these growth factors in rat skin wound and elucidate whether there are differences in the expression of these growth factors which might account for the delayed healing of the CO2 laser wounds compared to the scalpel as has been observed by some authors. Our results indicate that EGF, TGF-beta, PDGF, and bFGF are expressed and distributed in same areas of the early skin wound. The area of expression of these growth factors was associated with presence of wound inflammatory cells and wound fibroblasts. Our study found that there were no significant differences in the expression of growth factors in the majority of time points between the CO2 laser wounds and the scalpel wounds. (C) 1994 Wiley-Liss, Inc.