CELL-EXTRACTS OF CANDIDA-ALBICANS BLOCK ADHERENCE OF THE ORGANISMS TO ENDOTHELIAL-CELLS

被引：20

作者：

EDWARDS, JE

MAYER, CL

FILLER, SG

WADSWORTH, E

CALDERONE, RA

机构：

[1] UNIV CALIF LOS ANGELES, LOS ANGELES, CA 90024 USA

[2] GEORGETOWN UNIV, SCH MED, DEPT MICROBIOL, WASHINGTON, DC 20007 USA

[3] GEORGETOWN UNIV, SCH DENT, WASHINGTON, DC 20007 USA

来源：

INFECTION AND IMMUNITY | 1992年 / 60卷 / 08期

关键词：

D O I：

10.1128/IAI.60.8.3087-3091.1992

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Cell extracts of Candida albicans were fractionated by concanavalin A affinity chromatography. Eluted mannosylated proteins (fraction II) and nonbinding, nonmannosylated proteins (fraction I) were collected and assayed directly for inhibition of adherence of C. albicans to endothelium. Fraction II blocked blastospore adherence to endothelial cells. Fraction I blocked both blastospore and germ tube adherence to endothelial cells. Monoclonal antibody OKM-1 (anti-CR3) and an anti-C. albicans monoclonal antibody, CA-A (anti-CR2), reacted in Western blots with proteins from fraction I, suggesting the presence of the CR2- and CR3-like proteins that have been previously identified on C. albicans germ tubes.

引用

页码：3087 / 3091

页数：5

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