EFFECTS OF PH AND ORGANIC-SOLVENT ON CHROMATOGRAPHIC BEHAVIOR IN CAPILLARY ELECTROCHROMATOGRAPHY

被引：53

作者：

KITAGAWA, S ^{[1
]}

TSUDA, T ^{[1
]}

机构：

[1] NAGOYA INST TECHNOL,NAGOYA,AICHI 466,JAPAN

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1994年 / 6卷 / 02期

关键词：

ELECTROCHROMATOGRAPHY; EFFECT OF PH; EFFECT OF ORGANIC SOLVENT; CAPILLARY COLUMN; ELECTROOSMOSIS; ELECTROPHORETIC VELOCITY; APPLIED HIGH VOLTAGE;

D O I：

10.1002/mcs.1220060202

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Electrochromatography is demonstrated using a 50 mum i.d. capillary column. The separation of solutes due to their different mobilities is obtained by the application of high voltage along the column. The elution times of solutes are dependent on the pH and composition of the eluent. Both electrophoretic and electroosmotic flow velocities are almost constant between 30 and 90% methanol in the eluent, and they increase with more than 90% methanol. The pH dependence of the electroosmotic flow velocity may be related to the dissociation of unreacted silanol groups on the surface of the ODS-silica particles.

引用

页码：91 / 96

页数：6

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