IMPAIRED NATURAL-KILLER CYTOTOXICITY IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS IN GRAVES-DISEASE

被引:17
作者
MARAZUELA, M
VARGAS, JA
ALVAREZMON, M
ALBARRAN, F
LUCAS, T
DURANTEZ, A
机构
[1] CLIN PUERTA HIERO, SERV INTERNAL MED, MADRID, SPAIN
[2] CLIN PUERTA HIERO, SERV ENDOCRINOL, MADRID, SPAIN
[3] UNIV ALCALA DE HENARES, HOSP UNIV PRINCIPE ASTURIAS, MED SERV, MADRID, SPAIN
关键词
D O I
10.1530/eje.0.1320175
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We studied the natural killer (NK) activity of peripheral blood mononuclear cells (PBMC) in patients with Graves' disease (GD). Peripheral blood mononuclear cells from 20 untreated hyperthyroid patients with GD showed a significantly reduced NK activity against 51 Cr-labeled K562 cells (33.9 +/- 15.9%), while in 32 euthyroid patients under antithyroid drug therapy, NK activity was similar to that of controls (46.9 +/- 17.3 and 49.9 +/- 20.2%, respectively). Furthermore, normalization of thyroid function with antithyroid drugs was associated with a significant increase and normalization of NK activity during the follow-up of nine GD patients (from 29.2 +/- 17.9 to 48.1 +/- 16.5%). This phenomenon could not be ascribed to a defective number of NK cells because the amounts of CD56+ and CD16+ cells in PBMC from both hyperthyroid and euthyroid GD patients were within normal ranges. Natural killer activity of PBMC from patients with toxic multinodular goiter was similar to that of normal controls (45 +/- 12.8 to 49.9 +/- 20%). No correlation was found between natural killer activity and serum levels of free thyroxine, TSH-inhibitory immunoglobulins, thyroidal antibodies to thryoglobulin and thyroidal microsomal antigen, dose or duration of antithyroid drug therapy. Natural killer activity from both controls and GD patients was enhanced in vitro by addition of recombinant interleukin 2 (IL-2), reaching control levels in hyperthyroid patients. These abnormalities were not associated with a defective IL-2 production by T cells, nor with a decreased IL-2R expression. We conclude that in untreated Graves' disease there is a decrease in NK cell activity in PBMC, probably dependent on the autoimmune process. Possible biological and clinical implications are discussed.
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页码:175 / 180
页数:6
相关论文
共 29 条
[1]   CHARACTERIZATION OF HUMAN GRANULAR LYMPHOCYTE SUBPOPULATIONS EXPRESSING HNK-1 (LEU-7) AND LEU-11 ANTIGENS IN THE BLOOD AND LYMPHOID-TISSUES FROM FETUSES, NEONATES AND ADULTS [J].
ABO, T ;
MILLER, CA ;
BALCH, CM .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1984, 14 (07) :616-623
[2]  
ALVAREZMON M, 1989, ACTA HAEMATOL-BASEL, V81, P91
[3]   PERIPHERAL LYMPHOCYTES-K IN AUTOIMMUNE THYROID-DISEASE - DECREASE IN GRAVES-DISEASE AND INCREASE IN HASHIMOTOS DISEASE [J].
AMINO, N ;
MORI, H ;
IWATANI, Y ;
ASARI, S ;
IZUMIGUCHI, Y ;
MIYAI, K .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1982, 54 (03) :587-591
[4]   ANTIBODY-DEPENDENT CELL-MEDIATED CYTO-TOXICITY AGAINST HUMAN THYROID-CELLS IN HASHIMOTOS THYROIDITIS BUT NOT GRAVES-DISEASE [J].
BOGNER, U ;
SCHLEUSENER, H ;
WALL, JR .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1984, 59 (04) :734-738
[5]  
CALDER EA, 1976, CLIN EXP IMMUNOL, V25, P17
[6]  
DEMON MA, 1986, BLOOD, V67, P228
[7]   PRODUCTION OF AND RESPONSE TO INTERLEUKIN-2 IN GRAVES-DISEASE [J].
EISENSTEIN, Z ;
ENGELSMAN, E ;
WEISS, M ;
KALECHMAN, Y ;
SREDNI, B .
JOURNAL OF CLINICAL IMMUNOLOGY, 1988, 8 (05) :349-355
[8]   PERIPHERAL K CELLS IN GRAVES-DISEASE AND HASHIMOTOS THYROIDITIS IN RELATION TO CIRCULATING IMMUNE-COMPLEXES [J].
ENDO, Y ;
ARATAKE, Y ;
YAMAMOTO, I ;
NAKAGAWA, H ;
KURIBAYASHI, T ;
OHTAKI, S .
CLINICAL ENDOCRINOLOGY, 1983, 18 (02) :187-194
[9]  
FERGUSON MM, 1984, BRIT MED J, V288, P1004, DOI 10.1136/bmj.288.6422.1004-a
[10]   LYMPHOKINE - ACTIVATED KILLER CELL-ACTIVITY - CHARACTERISTICS OF EFFECTOR-CELLS AND THEIR PROGENITORS IN BLOOD AND SPLEEN [J].
HERBERMAN, RB ;
HISERODT, J ;
VUJANOVIC, N ;
BALCH, C ;
LOTZOVA, E ;
BOLHUIS, R ;
GOLUB, S ;
LANIER, LL ;
PHILLIPS, JH ;
RICCARDI, C ;
RITZ, J ;
SANTONI, A ;
SCHMIDT, RE ;
UCHIDA, A .
IMMUNOLOGY TODAY, 1987, 8 (06) :178-181