DIFFERENTIAL EXTRACTION OF ARTEMETHER AND ITS METABOLITE DIHYDROARTEMISININ FROM PLASMA AND DETERMINATION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：23

作者：

MUHIA, DK

MBERU, EK

WATKINS, WM

机构：

[1] WELLCOME TRUST RES LABS, NAIROBI, KENYA

[2] UNIV LIVERPOOL, DEPT PHARMACOL & THERAPEUT, LIVERPOOL L69 3BX, MERSEYSIDE, ENGLAND

[3] KENYA GOVT MED RES CTR, NAIROBI, KENYA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1994年 / 660卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0378-4347(94)00276-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the separation of artemether (ARM) from its metabolite dihydroartemisinin (DHA) and determination by HPLC. The basis of the separation is differential extraction of the drugs from plasma as a function of plasma pH. Hexane extracted ARM from basified plasma and both ARM and DHA from normal plasma. Derivatized extracts were chromatographed on a 5-mu m ODS column with water-acetonitrile (40:60) as mobile phase and detected at 254 nm. The method removes the need for expensive absorption cartridges (BondElut). Chromatography has been improved and the elution time shortened in comparison with previous methods.

引用

页码：196 / 199

页数：4

共 9 条

[1] GU H-M, 1980, Acta Pharmacologica Sinica, V1, P48
[2] DETERMINATION OF ARTEETHER IN BLOOD-PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION AFTER HYDROLYSIS WITH ACID
IDOWU, OR
EDWARDS, G
WARD, SA
ORME, ML
BRECKENRIDGE, AM
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 493 (01): : 125 - 136
[3] DECOMPOSITION REACTIONS OF ARTEETHER, A SEMISYNTHETIC DERIVATIVE OF QINGHAOSU (ARTEMISININ)
IDOWU, OR
MAGGS, JL
WARD, SA
EDWARDS, G
[J]. TETRAHEDRON, 1990, 46 (06) : 1871 - 1884
[4] JIANG J-R, 1983, Acta Pharmacologica Sinica, V4, P197
[5] QINGHAOSU (ARTEMISININ) - AN ANTIMALARIAL DRUG FROM CHINA
KLAYMAN, DL
[J]. SCIENCE, 1985, 228 (4703) : 1049 - 1055
[6] NIN XY, 1985, EUR J DRUG METAB PH, V10, P55
[7] SELECTIVE DETERMINATION, IN PLASMA, OF ARTEMETHER AND ITS MAJOR METABOLITE, DIHYDROARTEMISININ, BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION
THOMAS, CG
WARD, SA
EDWARDS, G
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 583 (01): : 131 - 136
[8] ZHANG YD, 1992, ACTA PHARMACOL SINIC, V17, P211
[9] ANALYSIS OF ARTESUNIC ACID AND DIHYDROQUINGHAOSU IN BLOOD BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH REDUCTIVE ELECTROCHEMICAL DETECTION
ZHOU, ZM
ANDERS, JC
CHUNG, H
THEOHARIDES, AD
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 414 (01): : 77 - 90

← 1 →