A NOVEL 57-KDA PEROXISOMAL MEMBRANE POLYPEPTIDE DETECTED BY MONOCLONAL-ANTIBODY (PXM1A/207B)

被引：20

作者：

IMANAKA, T ^{[1
]}

LAZAROW, PB ^{[1
]}

TAKANO, T ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED,DEPT CELL BIOL & ANAT,NEW YORK,NY 10029

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1062卷 / 02期

关键词：

PEROXISOME; PEROXISOMAL MEMBRANE; INTEGRAL MEMBRANE POLYPEPTIDE; MONOCLONAL ANTIBODY; CLOFIBRATE; (RAT LIVER);

D O I：

10.1016/0005-2736(91)90401-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

BALB / c mice were immunized with peroxisomal membranes prepared from rat liver. Spleen cells were fused with myeloma cells (P3/U1) and the hybridomas were selected using peroxisomal membranes. A monoclonal antibody (PXM1a / 207B) which recognized peroxisomal membranes was selected. Using the antibody, a novel 57 kDa polypeptide was identified in the peroxisomal membrane fraction. Immunoblot analysis of the subcellular fractions demonstrated that the 57 kDa polypeptide was present exclusively in peroxisomal membranes. The 57 kDa polypeptide was partially digested by trypsin and chymotrypsin under conditions where peroxisomal particles remained intact, indicating that the polypeptide is exposed to the cytosolic face of the peroxisomal membrane. The amount of 57 kDa polypeptide increased in parallel with proliferation of peroxisomes by administration of clofibrate.

引用

页码：264 / 270

页数：7

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