BIOSYNTHESIS AND APICAL LOCALIZATION OF THE UROKINASE RECEPTOR IN POLARIZED MDCK EPITHELIAL-CELLS

被引：17

作者：

LIMONGI, P

RESNATI, M

HERNANDEZMARRERO, L

CREMONA, O

BLASI, F

FAZIOLI, F

机构：

[1] UNIV MILAN,IST SCI SAN RAFFAELE,DIBIT,DEPT BIOL & TECHNOL RES,I-20132 MILAN,ITALY

[2] UNIV MILAN,DEPT GENET & MICROBIAL BIOL,I-20132 MILAN,ITALY

[3] UNIV TURIN,DIPARTIMENTO SCI MED,NOVARA BRANCH,NOVARA,ITALY

来源：

FEBS LETTERS | 1995年 / 369卷 / 2-3期

关键词：

UROKINASE RECEPTOR; GPI-ANCHORED PROTEIN; MDCK CELL;

D O I：

10.1016/0014-5793(95)00742-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biosynthesis and the surface localization of the urokinase plasminogen activator receptor (uPaR) were analysed in MDCK epithelial cells and in unpolarized fibroblasts. No differences were observed with respect to rate of synthesis, nature of precursors and time of surface appearance. uPAR,vas localized particularly at the focal and cell-cell contacts when expressed in fibroblasts. On the contrary, in MDCK cells uPAR was found mostly on the apical surface; in agreement with its localization, down-regulation of uPAR by the uPA-PAI-1 complex was observed only from the apical membrane.

引用

页码：207 / 211

页数：5

共 26 条

[1] BEHRENDT N, 1990, J BIOL CHEM, V265, P6453
[2] THE UROKINASE RECEPTOR - STRUCTURE, REGULATION AND INHIBITOR-MEDIATED INTERNALIZATION
BLASI, F
CONESE, M
MOLLER, LB
PEDERSEN, N
CAVALLARO, U
CUBELLIS, MV
FAZIOLI, F
HERNANDEZMARRERO, L
LIMONGI, P
MUNOZCANOVES, P
RESNATI, M
RIITTINEN, L
SIDENIUS, N
SORAVIA, E
SORIA, MR
STOPPELLI, MP
TALARICO, D
TEESALU, T
VALCAMONICA, S
[J]. FIBRINOLYSIS, 1994, 8 : 182 - 188
[3] MECHANISM OF MEMBRANE ANCHORING AFFECTS POLARIZED EXPRESSION OF 2 PROTEINS IN MDCK CELLS
BROWN, DA
CRISE, B
ROSE, JK
[J]. SCIENCE, 1989, 245 (4925) : 1499 - 1501
[4] INDUCTION OF CELL-MIGRATION BY PROUROKINASE BINDING TO ITS RECEPTOR - POSSIBLE MECHANISM FOR SIGNAL-TRANSDUCTION IN HUMAN EPITHELIAL-CELLS
BUSSO, N
MASUR, SK
LAZEGA, D
WAXMAN, S
OSSOWSKI, L
[J]. JOURNAL OF CELL BIOLOGY, 1994, 126 (01) : 259 - 270
[5] RECEPTOR-MEDIATED INTERNALIZATION AND DEGRADATION OF UROKINASE IS CAUSED BY ITS SPECIFIC INHIBITOR PAI-1
CUBELLIS, MV
WUN, TC
BLASI, F
[J]. EMBO JOURNAL, 1990, 9 (04) : 1079 - 1085
[6] THE UROKINASE RECEPTOR - PROTEIN-STRUCTURE AND ROLE IN PLASMINOGEN ACTIVATION AND CANCER INVASION
DANO, K
BEHRENDT, N
BRUNNER, N
ELLIS, V
PLOUG, M
PYKE, C
[J]. FIBRINOLYSIS, 1994, 8 : 189 - 203
[7] UROKINASE-TYPE PLASMINOGEN-ACTIVATOR AND ITS RECEPTOR - NEW TARGETS FOR ANTIMETASTATIC THERAPY
FAZIOLI, F
BLASI, F
[J]. TRENDS IN PHARMACOLOGICAL SCIENCES, 1994, 15 (01) : 25 - 29
[8] LINKAGE OF EXTRACELLULAR PLASMINOGEN-ACTIVATOR TO THE FIBROBLAST CYSTOSKELETON - COLOCALIZATION OF CELL-SURFACE UROKINASE WITH VINCULIN
HEBERT, CA
BAKER, JB
[J]. JOURNAL OF CELL BIOLOGY, 1988, 106 (04) : 1241 - 1247
[9] HOYERHANSEN G, 1992, J BIOL CHEM, V267, P18244
[10] LEBIVIC A, 1989, P NATL ACAD SCI USA, V86, P9313

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