EXPRESSION OF NA+-INDEPENDENT ISOLEUCINE TRANSPORT ACTIVITY FROM RAT-BRAIN IN XENOPUS-LAEVIS OOCYTES

被引：32

作者：

BROER, S

BROER, A

HAMPRECHT, B

机构：

[1] Physiologisch-Chemisches Institut der Universität, D-72076 Tübingen

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1192卷 / 01期

关键词：

AMINO ACID TRANSPORTER; OOCYTE; EXPRESSION; C6-BU-1; CELL; ASTROGLIAL CELL;

D O I：

10.1016/0005-2736(94)90147-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly(A)(+) RNA from C6-BU-1 rat glioma cells and rat astroglial cells induced isoleucine transport activity when injected into Xenopus laevis oocytes. The Na+-independent component of isoleucine transport was inhibited by leucine, phenylalanine and 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid (BCH) but neither by methylaminoisobutyric acid (MeAIB) nor lysine. A K-m value of approx. 100 mu M was determined for the Na+-independent transport of isoleucine. These data are in accordance with expression of a system L like transporter. By injection of size fractionated poly(A)(+) RNA a length of approx. 1.9 kb was determined for the pertinent mRNA.

引用

收藏

页码：95 / 100

页数：6

相关论文

共 42 条

[1] HIGH ACTIVITY OF CHOLINE-ACETYLTRANSFERASE INDUCED IN NEUROBLASTOMA - GLIA HYBRID CELLS [J].

AMANO, T ;

HAMPRECHT, B ;

KEMPER, W .

EXPERIMENTAL CELL RESEARCH, 1974, 85 (02) :399-408

[2] EXPRESSION OF AMINO-ACID TRANSPORT-SYSTEMS IN XENOPUS OOCYTES INJECTED WITH MESSENGER-RNA OF RAT SMALL-INTESTINE AND KIDNEY [J].

AOSHIMA, H ;

TOMITA, K ;

SUGIO, S .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1988, 265 (01) :73-81

[3]

ARRIZA JL, 1993, J BIOL CHEM, V268, P15329

[4]

BERTRAN J, 1993, J BIOL CHEM, V268, P14842

[5] EXPRESSION CLONING OF A CDNA FROM RABBIT KIDNEY CORTEX THAT INDUCES A SINGLE TRANSPORT-SYSTEM FOR CYSTINE AND DIBASIC AND NEUTRAL AMINO-ACIDS [J].

BERTRAN, J ;

WERNER, A ;

MOORE, ML ;

STANGE, G ;

MARKOVICH, D ;

BIBER, J ;

TESTAR, X ;

ZORZANO, A ;

PALACIN, M ;

MURER, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5601-5605

[6] STIMULATION OF SYSTEM Y+-LIKE AMINO-ACID-TRANSPORT BY THE HEAVY-CHAIN OF HUMAN 4F2 SURFACE-ANTIGEN IN XENOPUS-LAEVIS OOCYTES [J].

BERTRAN, J ;

MAGAGNIN, S ;

WERNER, A ;

MARKOVICH, D ;

BIBER, J ;

TESTAR, X ;

ZORZANO, A ;

KUHN, LC ;

PALACIN, M ;

MURER, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5606-5610

[7] EXPRESSION OF NA+-INDEPENDENT AMINO-ACID-TRANSPORT IN XENOPUS-LAEVIS OOCYTES BY INJECTION OF RABBIT KIDNEY CORTEX MESSENGER-RNA [J].

BERTRAN, J ;

WERNER, A ;

STANGE, G ;

MARKOVICH, D ;

BIBER, J ;

TESTAR, X ;

ZORZANO, A ;

PALACIN, M ;

MURER, H .

BIOCHEMICAL JOURNAL, 1992, 281 :717-723

[8] EFFECT OF PENTOBARBITAL ON AMINO-ACID AND UREA FLUX IN ISOLATED DOG BRAIN [J].

BETZ, AL ;

GILBOE, DD .

AMERICAN JOURNAL OF PHYSIOLOGY, 1973, 224 (03) :580-587

[9] TRIIODOTHYRONINE IS A HIGH-AFFINITY INHIBITOR OF AMINO-ACID-TRANSPORT SYSTEM-L1 IN CULTURED ASTROCYTES [J].

BLONDEAU, JP ;

BESLIN, A ;

CHANTOUX, F ;

FRANCON, J .

JOURNAL OF NEUROCHEMISTRY, 1993, 60 (04) :1407-1413

[10] NEUTRAL AMINO-ACID TRANSPORT IN ASTROCYTES - CHARACTERIZATION OF NA+-DEPENDENT AND NA+-INDEPENDENT COMPONENTS OF ALPHA-AMINOISOBUTYRIC-ACID UPTAKE [J].

BROOKES, N .

JOURNAL OF NEUROCHEMISTRY, 1988, 51 (06) :1913-1918

← 1 2 3 4 5 →