AN ENDOR AND ESEEM STUDY OF THE BLUE COPPER PROTEIN AZURIN

被引：46

作者：

COREMANS, JWA

VANGASTEL, M

POLUEKTOV, OG

GROENEN, EJJ

DENBLAAUWEN, T

VANPOUDEROYEN, G

CANTERS, GW

NAR, H

HAMMANN, C

MESSERSCHMIDT, A

机构：

[1] LEIDEN UNIV,LEIDEN INST CHEM,GORLAEUS LABS,2300 RA LEIDEN,NETHERLANDS

[2] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY

来源：

CHEMICAL PHYSICS LETTERS | 1995年 / 235卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/0009-2614(95)00114-J

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report electron-nuclear double-resonance experiments on a single crystal of azurin at 95 GHz and electron-spin-echo envelope-modulation experiments on frozen solutions of azurin and of the H117G mutant at 9 GHz. The hyperfine and quadrupole tensors of the two remote nitrogens of the histidine ligands of copper are assigned and discussed. A third nucleus is found to contribute to the echo-modulation spectrum and this probably concerns an amide nitrogen of the peptide backbone.

引用

页码：202 / 210

页数：9

共 30 条

[1] Structure and function of copper-containing proteins [J].