RAPID AND SAFE DETERMINATION OF HUMAN APOLIPOPROTEIN-E GENOTYPES BY MINIATURIZED SDS-PAGE IN NON-INSULIN-DEPENDENT DIABETES-MELLITUS

被引：9

作者：

CLAVEL, C

DURLACH, A

DURLACH, V

BIREMBAUT, P

机构：

[1] HOP MAISON BLANCHE,LAB POL BOUIN,UNITE BIOL CELLULAIRE,F-51100 REIMS,FRANCE

[2] HOP ROBERT DEBRE,MED CLIN,F-51100 REIMS,FRANCE

来源：

JOURNAL OF CLINICAL PATHOLOGY | 1995年 / 48卷 / 04期

关键词：

APOLIPOPROTEIN E; SDS-PAGE; NON-INSULIN DEPENDENT DIABETES MELLITUS;

D O I：

10.1136/jcp.48.4.295

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Aims-To present a non-isotopic procedure for the analysis of the different apolipoprotein E genotypes in normal subjects and patients with non-insulin dependent diabetes mellitus. Methods-Apolipoprotein E genotypes were detected following polymerase chain reaction and miniaturised sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) (PhastSystem Pharmacia). Results-The time taken from extraction of DNA from 6 mu l whole blood to the final result was eight hours. The allele frequencies in patients with diabetes mellitus in our series were similar to those found in the control subjects. Conclusions-SDS-PAGE is rapid, reliable and safe with few drawbacks, can be used as a routine procedure and may easily replace apolipoprotein E phenotyping.

引用

页码：295 / 299

页数：5

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