Alterations of superoxide dismutase iso-enzyme activity, content, and mRNA expression with aging in rat skeletal muscle

被引:67
作者
OhIshi, S
Kizaki, T
Yamashita, H
Nagata, N
Suzuki, K
Taniguchi, N
Ohno, H
机构
[1] NATL DEF MED COLL, DEPT INTERNAL MED 3, TOKOROZAWA, SAITAMA 359, JAPAN
[2] OSAKA UNIV, SCH MED, DEPT BIOCHEM, SUITA, OSAKA 565, JAPAN
关键词
aging; skeletal muscle; Mn-superoxide dismutase; Cu; Zn-superoxide dismutase;
D O I
10.1016/0047-6374(95)01637-F
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The alterations of superoxide dismutase iso-enzyme (Cu,Zn-SOD and Mn-SOD) activities, contents, and mRNA expressions with aging were studied in rat soleus muscle (SO) and extensor digitorum longus muscle (EDL). The activity and content of Cu,Zn-SOD in both muscles were significantly higher in old rats (24 months old) than in young rats (4 months old), whereas those of Mn-SOD showed no difference between young and old rats. After normalization to citrate synthase (CS) activity, however Mn-SOD/CS ratio in SO also showed the age-related increase. Moreover, the activities of other major antioxidant enzymes, glutathione peroxidase (GPX) and catalase (CAT), indicated age-related increases only in SO. As for the expressions of mRNAs for SOD iso-enzymes, that of Cu,Zn-SOD in either muscle showed no significant change with aging, unlike its activity and content, although that of Mn-SOD was decreased with aging only in EDL. Thus, aging appeared to raise the level of antioxidant enzyme system in rat skeletal muscle. However, the resistance of Cu,Zn-SOD and Mn-SOD to oxidative stress accompanied by aging was different, the former being obviously greater than the latter. Such changes also differed in muscle fiber type suggesting that fast-twitch fibers are more susceptible to age-related oxidative stress than slow-twitch fibers.
引用
收藏
页码:65 / 76
页数:12
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