A LARGE INCREASE IN ENZYME SUBSTRATE AFFINITY BY PROTEIN ENGINEERING

被引：132

作者：

WILKINSON, AJ

FERSHT, AR

BLOW, DM

CARTER, P

WINTER, G

机构：

[1] UNIV LONDON IMPERIAL COLL SCI & TECHNOL,DEPT PHYS,LONDON SW7 2AY,ENGLAND

[2] MRC,MOLEC BIOL LAB,CAMBRIDGE CB2 2QH,ENGLAND

来源：

NATURE | 1984年 / 307卷 / 5947期

关键词：

D O I：

10.1038/307187a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

引用

页码：187 / 188

页数：2

共 17 条

[1] TYROSYL-TRANSFER RNA-SYNTHETASE FORMS A MONONUCLEOTIDE-BINDING FOLD [J].

BHAT, TN ;

BLOW, DM ;

BRICK, P ;

NYBORG, J .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 158 (04) :699-709

[2] BUFFER GRADIENT GELS AND S-35 LABEL AS AN AID TO RAPID DNA-SEQUENCE DETERMINATION [J].

BIGGIN, MD ;

GIBSON, TJ ;

HONG, GF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13) :3963-3965

[3] RAPID SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES .7. SOLID-PHASE SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES BY A CONTINUOUS-FLOW PHOSPHOTRIESTER METHOD ON A KIESELGUHR-POLYAMIDE SUPPORT [J].

GAIT, MJ ;

MATTHES, HWD ;

SINGH, M ;

SPROAT, BS ;

TITMAS, RC .

NUCLEIC ACIDS RESEARCH, 1982, 10 (20) :6243-6254

[4] DIRECTED SELECTIVE PRESSURE ON A BETA-LACTAMASE TO ANALYZE MOLECULAR CHANGES INVOLVED IN DEVELOPMENT OF ENZYME FUNCTION [J].

HALL, A ;

KNOWLES, JR .

NATURE, 1976, 264 (5588) :803-804

[5] EVOLUTION OF A NEW ENZYMATIC FUNCTION BY RECOMBINATION WITHIN A GENE [J].

HALL, BG ;

ZUZEL, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (06) :3529-3533

[6]

HALL BG, 1978, GENETICS, V89, P453

[7] BINDING OF TYROSINE, ADENOSINE-TRIPHOSPHATE AND ANALOGS TO CRYSTALLINE TYROSYL TRANSFER-RNA SYNTHETASE [J].

MONTEILHET, C ;

BLOW, DM .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 122 (04) :407-417

[8] MOLECULAR-BASIS OF ALTERED ENZYME SPECIFICITIES IN A FAMILY OF MUTANT AMIDASES FROM PSEUDOMONAS-AERUGINOSA [J].

PATERSON, A ;

CLARKE, PH .

JOURNAL OF GENERAL MICROBIOLOGY, 1979, 114 (SEP) :75-85

[9] AMINO-ACID ACTIVATION IN CRYSTALLINE TYROSYL-TRANSFER RNA-SYNTHETASE FROM BACILLUS-STEAROTHERMOPHILUS [J].

RUBIN, J ;

BLOW, DM .

JOURNAL OF MOLECULAR BIOLOGY, 1981, 145 (03) :489-500

[10] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

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