MEDIA CARBON INDUCTION OF EXTRACELLULAR CELLULASE ACTIVITIES IN NEOCALLIMASTIX-FRONTALIS ISOLATE EB188

被引：25

作者：

BARICHIEVICH, EM

CALZA, RE

机构：

[1] Animal Sciences Department, Washington State University, Pullman, 99164, WA

来源：

CURRENT MICROBIOLOGY | 1990年 / 20卷 / 04期

关键词：

D O I：

10.1007/BF02089422

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Extracellular cellulase induction in the ruminal fungus Neocallimastix frontalis isolate EB188 was followed. Glucose media-established cultures produced cellulase when switched to a variety of cellulose-containing media. High levels of cellulase and xylanase activities were present in cultures switched to sigma cell 100, solka floc, avicel, sisal fiber, and wheat straw, but not those switched to glucose, carboxymethylcellulose, or wood chips. Several assay substrates were used to show differential cellulase induction as well as β-glucosidase activity. Cellulases hydrolyzed short oligosaccharides and released glucose from insoluble cellulose. Cellobiase activity was also indicated. Cellulase activity tolerated brief exposure to high temperature, was insensitive to certain metal ions, and possessed pH optima between 5.0 and 6.5. © 1990 Springer-Verlag New York Inc.

引用

页码：265 / 271

页数：7

共 45 条

[1] RUMEN FUNGI - MORPHOLOGICAL TYPES FROM GEORGIA CATTLE AND THE ATTACK ON FORAGE CELL-WALLS [J].