ESTIMATION OF THE PULMONARY MICROVASCULAR REFLECTION COEFFICIENT TO PROTEIN IN DOGS

We used a new technique to estimate the pulmonary microvascular membrane reflection coefficient to plasma protein (sigma-d) in anesthetized dogs. In five animals we continuously weighed the lower left lung lobe and used a left atrial balloon to increase the pulmonary microvascular pressure (P(c)). We determined the relationship between the rate of edema formation (S) and P(c) and estimated the fluid filtration coefficient (K(f)) as DELTA-S/DELTA-P(c). From the S vs. P(c) relationship and K(f), we estimated the P(c) at which S/K(f) = 10 mmHg for each dog. This pressure (P10) was 38.0 +/- 5.8 (SD) mmHg, and the plasma protein osmotic pressure (pi-c) was 14.9 +/- 3.7 mmHg. In five additional dogs in which we decreased pi-c to 2.9 +/- 1.7 mmHg, P10 = 27.2 +/- 2.6 mmHg. The P10 vs. pi-c regression line fit to the data from all 10 dogs was P10 = 0.92 pi-c + 24.4 mmHg (r = 0.88). We estimated sigma-d from the slope of the regression line as sigma-d = square-root DELTA-P10/DELTA-pi-c. With this technique, we estimated that, with 95% probability, sigma-d lies between 0.72 and unity. This is higher than most previous sigma-d estimates.