ANALYSIS OF THE PEIE PROMOTER IN ERWINIA-CHRYSANTHEMI EC16

被引：18

作者：

GOLD, S ^{[1
]}

NISHIO, S ^{[1
]}

TSUYUMU, S ^{[1
]}

KEEN, NT ^{[1
]}

机构：

[1] SHIZUOKA UNIV,GRAD SCH AGR,OYA,SHIZUOKA 422,JAPAN

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1992年 / 5卷 / 02期

关键词：

GENE REGULATION; PECTIC ENZYMES;

D O I：

10.1094/MPMI-5-170

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pelE gene of Erwinia chrysanthemi strain EC16 encodes an extracellular pectate lyase protein that is important in virulence on plants. Control of pelE expression is complex, because the gene is regulated by catabolite repression, substrate induction, and growth-phase inhibition. A Tn7-lux reporter gene system was employed to define DNA sequences comprising the pelE promoter. When EC16 cells were grown on medium containing sodium polypectate, pelE transcriptional start sites were observed only at 95 and 96 bases upstream of the translational start site. However, DNA sequences required for pelE expression were also shown by deletion analysis to reside between 196 and 215 base pairs upstream of the translational start site. In addition to these upstream elements, two putative operator sequences that interact with negative regulatory factors occurred downstream of the transcriptional start. Finally, deletion of three bases from a putative catabolite gene activator protein binding site in the pelE promoter eliminated activity. The data demonstrate that the pelE promoter is complex and suggest that it interacts with several regulatory proteins.

引用

页码：170 / 178

页数：9