CELLULAR-LOCALIZATION AND DIFFERENTIAL DISTRIBUTION OF GABA(A) RECEPTOR SUBUNIT PROTEINS AND MESSENGER-RNAS WITHIN HYPOTHALAMIC MAGNOCELLULAR NEURONS

被引:60
作者
FENELON, VS [1 ]
SIEGHART, W [1 ]
HERBISON, AE [1 ]
机构
[1] UNIV VIENNA,PSYCHIAT KLIN,DEPT BIOCHEM PSYCHIAT,VIENNA,AUSTRIA
基金
英国惠康基金;
关键词
D O I
10.1016/0306-4522(94)00402-Q
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The inhibitory neurotransmitter GABA plays an important role in regulating the activity of magnocellular oxytocin and vasopressin neurons located in the supraoptic and paraventricular nuclei through occupancy of GABA(A) receptors. However, the GABA(A) receptor is a hetero-oligomeric protein comprised of different subunits and the subunit types expressed in a given receptor complex appear critical for its sensitivity to GABA, benzodiazepines and/or steroids. Thus, in order to understand fully the GABAergic control of oxytocin and vasopressin secretion, definition of the GABA(A) receptors synthesized by magnocellular neurons in the supraoptic and paraventricular nuclei is required. In the supraoptic nucleus, antibodies directed against the alpha(1), alpha(2), and beta(2/3) subunits of the GABA(A) receptor revealed similar strong antigen distribution on all magnocellular neurons. Using sequential double-immunoperoxidase staining, immunoreactivity for ail three subunits was observed on both oxytocin and vasopressin neurons of the supraoptic nucleus. In contrast, only alpha(2) subunit immunoreactivity was detected on the cell bodies of oxytocin and vasopressin neurons in the paraventricular nucleus. No sex differences were detected. In situ hybridization experiments using S-35-labelled oligonucleotides showed that all supraoptic neurons expressed alpha(1), alpha(2) and beta(2), subunit messenger RNA transcripts while magnocellular neurons in the paraventricular nucleus were only enriched in alpha(2) subunit messenger RNA. Quantitative analysis showed that the expression of alpha(1) and beta(2) subunit messenger RNAs in the paraventricular nucleus was half that observed in the supraoptic nucleus while expression of beta(3) subunit messenger RNA was very low in both nuclei. These results show that all oxytocin and vasopressin neurons located in the supraoptic nucleus synthesize and express alpha(1), alpha(2) and beta(2), subunits of the GABA(A) receptor while those in the paraventricular nucleus are only immunoreactive for the alpha(2) subunit. These observations suggest, therefore, that at least two pharmacologically distinct GABA(A) receptor isoforms exist on supraoptic neurons and that these are different to those expressed by paraventricular magnocellular cells. Thus, in addition to providing a definition of the subunits likely to form specific GABA(A) receptor isoforms on magnocellular neurons, this study gives direct evidence for GABA(A) receptor heterogeneity between supraoptic and paraventricular neurons, but not between oxytocin and vasopressin cells.
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页码:1129 / 1143
页数:15
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