A NOVEL ACTIVITY OF ESCHERICHIA-COLI URACIL DNA N-GLYCOSYLASE EXCISION OF ISODIALURIC ACID (5,6-DIHYDROXYURACIL), A MAJOR PRODUCT OF OXIDATIVE DNA-DAMAGE, FROM DNA

被引：41

作者：

ZASTAWNY, TH

DOETSCH, PW

DIZDAROGLU, M

机构：

[1] NIST,CHEM SCI & TECHNOL LAB,GAITHERSBURG,MD 20899

[2] MED ACAD POLAND,DEPT CLIN BIOCHEM,PL-85094 BYDGOSZCZ,POLAND

[3] EMORY UNIV,SCH MED,DEPT BIOCHEM,ATLANTA,GA 30322

来源：

FEBS LETTERS | 1995年 / 364卷 / 03期

关键词：

OXIDATIVE DNA DAMAGE; HYDROXYL RADICAL; DNA REPAIR; MODIFIED BASE; ISODIALURIC ACID;

D O I：

10.1016/0014-5793(95)00400-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a novel activity of E. coli uracil DNA N-glycosylase (UNG) that excises isodialuric acid from DNA, Isodialuric acid is formed in DNA as a major oxidative product of cytosine, DNA substrates, which were prepared by gamma-irradiation, were incubated with UNG. Following precipitation of DNA, analyses of pellets and supernatant fractions by gas chromatography/mass spectrometry showed an efficient excision of isodialuric acid from DNA by UNG. None of the other 15 identified DNA base lesions was excised, The excision of isodialuric acid indicates that the non-aromaticity of a substrate may not be a limiting factor for UNG.

引用

页码：255 / 258

页数：4

共 24 条

[1] BASE MODIFICATIONS IN PLASMID DNA CAUSED BY POTASSIUM-PERMANGANATE [J].