CRYOENZYMOLOGY OF TRYPSIN - A DETAILED KINETIC-STUDY OF THE TRYPSIN-CATALYZED HYDROLYSIS OF N-ALPHA-BENZYLOXYCARBONYL-L-LYSINE PARA-NITROPHENYL ESTER AT LOW-TEMPERATURES

被引：17

作者：

MALTHOUSE, JPG ^{[1
]}

SCOTT, AI ^{[1
]}

机构：

[1] TEXAS A&M UNIV,CTR BIOL NMR,DEPT CHEM,COLLEGE STN,TX 77843

来源：

BIOCHEMICAL JOURNAL | 1983年 / 215卷 / 03期

关键词：

D O I：

10.1042/bj2150555

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：555 / 563

页数：9

共 36 条

[1]

ANTONINI E, 1981, J BIOL CHEM, V256

[2] STEADY-STATE AND PRE-STEADY-STATE KINETICS OF THE TRYPSIN-CATALYZED HYDROLYSIS OF "ALPHA-CBZ-L-LYSINE-PARA-NITROPHENYL ESTER [J].

ASCENZI, P ;

MENEGATTI, E ;

BORTOLOTTI, F ;

GUARNERI, M ;

ANTONINI, E .

BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 658 (01) :158-164

[3] IDENTITY OF RATES OF DEACYLATION OF NONIONIC ACYL-ALPHA-CHYMOTRYPSINS + ACYL-TRYPSINS [J].

BENDER, ML ;

KEZDY, FJ ;

KILLHEFFER, JV .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1964, 86 (23) :5330-&

[4] FORMATION OF AN ACYL-ENZYME INTERMEDIATE IN ALPHA-CHYMOTRYPSIN-CATALYZED HYDROLYSES OF NON-LABILE TRANS-CINNAMIC ACID ESTERS [J].

BENDER, ML ;

ZERNER, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1962, 84 (13) :2550-&

[5] DETERMINATION OF CONCENTRATION OF HYDROLYTIC ENZYME SOLUTIONS - ALPHA-CHYMOTRYPSIN TRYPSIN PAPAIN ELASTASE SUBTILISIN AND ACETYLCHOLINESTERASE [J].

BENDER, ML ;

BEGUECAN.ML ;

BLAKELEY, RL ;

BRUBACHER, LJ ;

FEDER, J ;

GUNTER, CR ;

KEZDY, FJ ;

KILLHEFFER, JV ;

MARSHALL, TH ;

MILLER, CG ;

ROESKE, RW ;

STOOPS, JK .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1966, 88 (24) :5890-+

[6]

BENDER ML, 1965, J AM CHEM SOC, V87, P4935

[7] REFINED CRYSTAL-STRUCTURE OF BOVINE BETA-TRYPSIN AT 1.8 A RESOLUTION .2. CRYSTALLOGRAPHIC REFINEMENT, CALCIUM-BINDING SITE, BENZAMIDINE BINDING-SITE AND ACTIVE-SITE AT PH 7.0 [J].

BODE, W ;

SCHWAGER, P .

JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (04) :693-717

[8] PH-DEPENDENCE OF 2ND-ORDER RATE CONSTANTS OF ENZYME MODIFICATION MAY PROVIDE FREE-REACTANT PKA VALUES [J].

BROCKLEHURST, K .

BIOCHEMICAL JOURNAL, 1977, 167 (03) :859-862

[9] ACCURACY OF REFINED PROTEIN STRUCTURES - COMPARISON OF 2 INDEPENDENTLY REFINED MODELS OF BOVINE TRYPSIN [J].

CHAMBERS, JL ;

STROUD, RM .

ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL SCIENCE, 1979, 35 (AUG) :1861-1874

[10] P-NITROPHENYL-P'-GUANIDINOBENZOATE HCL - A NEW ACTIVE SITE TITRANT FOR TRYPSIN [J].

CHASE, T ;

SHAW, E .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1967, 29 (04) :508-&

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