RAPID IDENTIFICATION OF PROTEINS

被引:51
作者
SHAW, G
机构
关键词
AMINO ACID COMPOSITION; MOLECULAR WEIGHT; ISOELECTRIC POINT; PROTEIN ANALYSIS;
D O I
10.1073/pnas.90.11.5138
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The amino acid composition, molecular weight, and isoelectric point of a protein can all be easily and economically determined by current electrophoretic techniques. A method which uses such easily obtained data to identify proteins is described. A computer program first corrects for systematic errors in amino acid quantitation and then searches the current sequence database for proteins with amino acid compositions similar to the corrected values, taking into account the reliability of determination of each amino acid. The program also provides the calculated molecular weight, isoelectric point, and name of each candidate, providing three further independent criteria for protein identification. The program is surprisingly sensitive, and the composition data alone, if of good quality, usually suggest the correct protein as a strong candidate if it or a close homologue is present in the database. Further studies show that proteins in the current database have amino acid compositions distinct enough to allow this method to be generally applicable. The method is a quick and cost-effective first step in protein characterization and should become increasingly useful as the number of fully sequenced proteins continues to rise.
引用
收藏
页码:5138 / 5142
页数:5
相关论文
共 12 条
[1]   CHARACTERIZATION OF A NOVEL 66-KD SUBUNIT OF MAMMALIAN NEUROFILAMENTS [J].
CHIU, FC ;
BARNES, EA ;
DAS, K ;
HALEY, J ;
SOCOLOW, P ;
MACALUSO, FP ;
FANT, J .
NEURON, 1989, 2 (05) :1435-1445
[2]   IDENTIFICATION OF MOUSE-BRAIN PROTEINS AFTER TWO-DIMENSIONAL ELECTROPHORESIS AND ELECTROBLOTTING BY MICROSEQUENCE ANALYSIS AND AMINO-ACID COMPOSITION ANALYSIS [J].
ECKERSKORN, C ;
JUNGBLUT, P ;
MEWES, W ;
KLOSE, J ;
LOTTSPEICH, F .
ELECTROPHORESIS, 1988, 9 (12) :830-838
[3]   THE PREDICTED AMINO-ACID-SEQUENCE OF ALPHA-INTERNEXIN IS THAT OF A NOVEL NEURONAL INTERMEDIATE FILAMENT PROTEIN [J].
FLIEGNER, KH ;
CHING, GY ;
LIEM, RKH .
EMBO JOURNAL, 1990, 9 (03) :749-755
[4]  
GHARAHDAGHI F, 1992, TECHNIQUES PROTEIN C, V3, P249
[5]  
LEGENDRE N, 1990, Biotechniques, V9, P788
[6]  
Matsudaira P, 1989, PRACTICAL GUIDE PROT
[7]  
OZOLS J, 1990, METHOD ENZYMOL, V182, P587
[8]   BUNDLING AND CROSS-LINKING OF INTERMEDIATE FILAMENTS OF THE NERVOUS-SYSTEM [J].
SHAW, G ;
HOU, ZC .
JOURNAL OF NEUROSCIENCE RESEARCH, 1990, 25 (04) :561-568
[9]   IDENTIFICATION OF PROTEINS IN SEQUENCE DATABASES FROM AMINO-ACID-COMPOSITION DATA [J].
SIBBALD, PR ;
SOMMERFELDT, H ;
ARGOS, P .
ANALYTICAL BIOCHEMISTRY, 1991, 198 (02) :330-333
[10]  
Strydom D, 1992, TECHNIQUES PROTEIN C, VIII, P261