ANALYSIS OF URACIL IN DNA BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY

被引：62

作者：

BLOUNT, BC ^{[1
]}

AMES, BN ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY, DEPT MOLEC & CELL BIOL, BERKELEY, CA 94720 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 219卷 / 02期

关键词：

D O I：

10.1006/abio.1994.1257

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive method using gas chromatography and negative chemical ionization mass spectrometry for the detection of uracil in DNA is described. Uracil DNA glycosylase is used to specifically cleave uracil from DNA. Once removed, uracil is derivatized with 3,5-bis(trifluoromethyl)benzyl bromide and the sample components are separated with gas chromatography. Negative chemical ionization mass spectrometry is used to quantitatively detect as little as 1 pg of uracil per 100 pg DNA. This assay is 10 times more sensitive than previously described quantitative methods for the detection of uracil in DNA. We apply this method as part of a project to determine why folic acid deficiency causes DNA breaks. We demonstrate that inhibition of folic acid metabolism induces an accumulation of uracil in DNA. (C) 1994 Academic Press, Inc.

引用

页码：195 / 200

页数：6

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