GENE-MAPPING IN LENTIL WITH RECOMBINANT INBRED LINES

Homozygous recombinant lines developed from interspecific Lens hybrids were used to map isozyme and morphological markers in lentil. F-3 families from eight interspecific (L. culinaris x L. orientalis) lentil crosses were advanced to the F-6 by single seed descent to develop eight sets of recombinant inbred lines. Each of the eight sets of lines was as sayed for parental alleles at 2-8 isozyme and four morphological trait loci. Single locus goodness of fit to the expected 1:1 ratio was determined for each of the marker loci, and linkage relationships among the marker loci were determined. Six linkage groups were identified, and included a total of 17 isozyme loci and four morphological trait loci. We confirmed previously reported linkages among isozyme loci and morphological trait loci (Gs: Aat-p, Pi:Gal-1, Yc:Pgm-p, Aat-c:Me-2, Aat-mb:Skdh, Fk:Pgd-p), and identified three additional linkage groups (Gh:Gal-l, Gal-2:Aat-mb:Skdh, and Skdh:Aldo:Nag). The genetic map of lentil was expanded and confirmed based on these linkages, and a database was generated that can be used to add additional markers to the gene map.