COMPLETE NUCLEOTIDE-SEQUENCE OF THE AZOTOBACTER-VINELANDII NITROGENASE STRUCTURAL GENE-CLUSTER

被引:148
作者
BRIGLE, KE
NEWTON, WE
DEAN, DR
机构
[1] BATTELLE CF KETTERING RES LAB, POB 268, 150 E S COLL ST, YELLOW SPRINGS, OH 45387 USA
[2] USDA ARS, WESTERN REG RES CTR, ALBANY, CA 94710 USA
关键词
D O I
10.1016/0378-1119(85)90255-0
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
DNA fragments coding for the structural genes for Azotobacter vinelandii nitrogenase have been isolated and sequenced. These genes, nifH, nifD and nifK, code for the iron (Fe) protein and the .alpha. and .beta. subunits of the molybdenum-iron (MoFe) protein, respectively. They are arranged in the order: promoter:nifH:nifD:nifK. There are 129 nucleotides separating nifH and nifD and 101 nucleotides separating nifD and nifK. The amino acid (aa) sequences deduced from the nucleotide sequences are discussed in relation to the prosthetic group-binding regions of the nifHDK-encoded polypeptides.
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页码:37 / 44
页数:8
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